Molecular Detection of Listeria Species Isolated From Raw Milk with Special Reference to Virulence Determinants and Antimicrobial Resistance in Listeria monocytogenes
Molecular Detection of Listeria Species Isolated From Raw Milk with Special Reference to Virulence Determinants and Antimicrobial Resistance in Listeria monocytogenes
Hams M.A. Mohamed1*, Katreen K.G.2, M.W. Abd Al-Azeem1, Faysal A. Wasel2, Ahmed M. Abd-Eldayem3
(a):Amplified profile of Listeria spp. DNA positive for iap gene at 1500 bp. , Lane(1):Gel Pilot 100 bp ladder , Lane2: positive control, Lane3:Negative control, Lane 4,5,6,7,8,9,10,11 positive isolates. (b): Amplified profile of Listeria monocytogenes. DNA positive for species specific 16S rRNA gene at 1200 bp., Lane(1):Gel Pilot 100 bp ladder, Lane2: positive control, Lane3:Negative control, Lane 4,5,6,7,8,9,10,11positive isolates.
Phylogenetic Tree of the Entire Nucleotide Sequence of 16SrRNA Gene of Our Listeria spp. Isolates Compared with Reference Strains Regained from Gene Bank
Biofilm formation by L. monocytogenes isolates
Relationship between Multidrug resistance and biofilm formation among L.monocytogenes isolates
PCR products of the amplified hlyA, inlA and inB genes in L.monocytogenes were electrophoresed on an agarose gel.(a)amplified prodcut of hlyA gene at 174bp, lane1: Gel Pilot 100 bp ladder, lane2:postive control, laneL:3negative control, lane:4-11postive isolates. (b) amplified prodcut of inlA gene at 800bp,lane1: Gel Pilot 100 bp ladder, lane2: postive control, lane3:negative control, lane (5,9,10 and 11) postive isolates. (c) amplified prodcut of inB gene at 343 bp,lane1: Gel Pilot 100 bpladder, lane 2: postive control, lane 3: negative control, lane: 4-11postive isolates.
PCR products of the amplified mefA, aad6, tetM and ampC genes in L.monocytogenes were electrophoresed on an agarose gel.(a)amplified prodcut of mefA gene at 348bp, lane1: Gel Pilot 100 bp ladder, lane 2:postive control, lane3: negative control, lane:5,6 and9 postive isolates. (b) amplified prodcut of aad6 gene at 978bp,lane1: Gel Pilot 100 bp ladder, lane2:postive control, lane(3-10)postive isolates, lane11:negative control. (c) amplified prodcut of tetM gene at 405bp, lane1: Gel Pilot 100 bp ladder, lane2: postive control, lane3: negative control, lane: 4-11postive isolates.(d) amplified prodcut of ampC gene at 550bp,lane1: Gel Pilot 100 bp ladder, lane2: postive control,lane3: negative control, lane:4-11postive isolates
