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Isolation and Identification of Mycoplasma mycoides Subspecies mycoides from the Ear Canal of Cattle in Plateau State, North-Central Nigeria

Isolation and Identification of Mycoplasma mycoides Subspecies mycoides from the Ear Canal of Cattle in Plateau State, North-Central Nigeria

Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa

Bacterial Vaccine Production Division; 2Bacterial Research Division; Applied Biotechnology Division, National Veterinary Research Institute, Vom, Plateau State, Nigeria; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria; 5Pan African Veterinary Vaccine Centre, Addis Ababa, Ethiopia.

 

drpaulidokoankeli@yahoo.com

Figure 1

Map of Plateau State showing Local Government Areas sampled (Source: National Bureau of Statistics)

Figure 2

Photomicrograph of Mycoplasma mycoides subspecies mycoides (Mmm) colonies isolated from ear swab samples from cattle on mycoplasma agar. Note: raised dense centres and lighter peripheries (arrows), x35 

Figure 3

Mmm-specific PCR showing 574 bp: Lane M is the molecular marker (50 bp ladder), Lane 1, 2, 3, for the three positive Mmm, respectively, Lane +ve, -ve for negative and positive (T1/44 vaccine strain) controls 

Figure 4

Mmm-specific PCR showing 1.1 kb amplicon sizes ran in duplicates: Lane M is the molecular marker (1kp plus ladder), Lane 1A-1B, 2A-2B, 3A-3B, for the three positives, respectively, Lane –ve, +ve for negative and positive (T1/44 vaccine strain) controls 

Veterinary Sciences: Research and Reviews

June

Vol. 7, Iss. 1, Pages 1-91

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