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Isolation of Lactoferrin from Camel Milk through Fast Protein Liquid Chromatography and its Antagonistic Activity against Escherichia coli and Staphylococcus aureus

Isolation of Lactoferrin from Camel Milk through Fast Protein Liquid Chromatography and its Antagonistic Activity against Escherichia coli and Staphylococcus aureus

Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2

1National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan
2Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan

*      Corresponding author: imbn218@yahoo.com

Fig 1

Fast Protein Liquid chromatogram of Lf isolated from Camel milk.

Fig 2

SDS-PAGE of lactoferrin isolated from camel milk through fast protein liquid chromatography.

Fig 3

Effect of different conc. of CLf against E. coli and S. aureus.T0(1), Positive control: standard antibiotic (ampicillin and streptomycin); T1(2), Negative control (disc without Lf); T1, 1ppm; T2, 10 ppm; T3, 50 ppm; T4, 100 ppm; T5, 500 ppm; T6, 1000 ppm; T7, 2000 ppm; T8, 4000 ppm

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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