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Isolation and Genetic Characterization of Hobi- Like Pestivirus Circulating in Egyptian Cattle during 2019-2020

Isolation and Genetic Characterization of Hobi- Like Pestivirus Circulating in Egyptian Cattle during 2019-2020

Zeinab R. Aboezz1*, Ayman S. El-Habbaa1, Rania S. El-Mohamady2, Samia A. Elnagar2, Ehab M. El-Nahas1

1Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor 13736, Kalubia, Egypt; 2Viral Diseases Research Unit, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), Giza, Egypt.


*Correspondence | Zeinab Aboezz, Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor 13736, Kalubia, Egypt; Email: [email protected]

Figure 1:

Neighbor-joining Phylogenetic tree for partial 5` UTR nucleotide sequence of BVDV- Egypt-020-1ncp (MZ873104) and BVDV-Egypt-020-3ncp (MZ873105). strains compared with other BVDV-1, BVDV-2 and BVDV-3 reference sequence obtained from GenBank database. The scale bar represents the number of substitutions per nucleotide. Egypt-020-1ncp and BVDV-Egypt-020-3ncp strains from the present study are indicated by black circle.

Figure 2:

Nucleotide variations in 288bp 5UTR products of BVDV-Egypt-020-1ncp (original serum sample) and BVDV-Egypt-020- 2ncp (third passage on MDBK cells from serum). Dots or hide refer to nucleotide similarities while box refer to nucleotide differences.

Figure 3:

Nucleotide variations in 288bp 5UTR products of BVDV-Egypt-020-1ncp (original serum sample) and BVDV-Egypt-020- 3ncp (original ovary sample). Dots or hide refer to nucleotide similarities while box refer to nucleotide differences.

Advances in Animal and Veterinary Sciences

December

Vol. 12, Iss. 12, pp. 2301-2563

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