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Insight into the Mechanism of Doxorubicin-induced Nephrotoxicity through Gene Expression Analysis of Oxidative Stress, Kidney Injury and Inflammation Markers

Insight into the Mechanism of Doxorubicin-induced Nephrotoxicity through Gene Expression Analysis of Oxidative Stress, Kidney Injury and Inflammation Markers

Uzma Jabeen1, Asmat Salim2*, Irfan Khan2, Nadia Naeem3 and Rubina Mushtaq4

1Department of Biochemistry, Federal Urdu University of Arts, Science and Technology, Gulshan-e-Iqbal Campus Karachi, Karachi-75300, Pakistan
2Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan
3Dow University of Health Sciences, Ojha Campus, Gulzar‑e‑Hijri, Suparco Road, KDA Scheme‑33, Karachi, Pakistan
4Department of Zoology, Federal Urdu University of Arts, Science and Technology, Gulshan-e-Iqbal Campus Karachi, Karachi-75300, Pakistan
 
* Corresponding author: asmat.salim@iccs.edu

Fig. 1.

Agrose gels showing RT-PCR expression analysis of the oxidative stress induced genes, glutathione peroxidase-1(Gpx1), NAD(P)H quinone dehydrogenase 1 (Nqo1), 24-dehydrocholesterol reductase (Dhcr24), dual oxidase 2 (Duox2), and isocitrate dehydrogenase-1 (Idh1) in the kidney tissue following DOX injection (3 mg / kg; i.p.) in SD rats. All data are expressed as the means ± standard error of the mean. Data were subjected to Student’s t-test and values corresponding to P < 0.05 is considered statistically significant.

Fig. 3.

Agrose gels showing RT-PCR gene expression analysis of the anti-inflammatory cytokine, interleukin-10 (IL10) in the kidney tissue following DOX injection (3 mg / kg; i.p.) in SD rats. All data are expressed as the means ± standard error of the mean. Data were subjected to Student’s t-test and values corresponding to P < 0.05 is considered statistically significant.

Fig. 2.

Agrose gels showing RT-PCR gene expression analysis of the kidney injury biomarkers, kidney injury molecule-1 (KIM-1) and osteopontin (OPN) in the kidney tissue following DOX injection (3 mg / kg; i.p.) in SD rats. All data are expressed as the means ± standard error of the mean. Data were subjected to Student’s t-test and values corresponding to P < 0.05 is considered statistically significant.

Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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