Inhibition of Oxidative Stress and Inflammation by Fisetin Ameliorates Heat Stress-Induced Intestinal Injury in Rats
Inhibition of Oxidative Stress and Inflammation by Fisetin Ameliorates Heat Stress-Induced Intestinal Injury in Rats
Kang Cheng1*, Zhihua Song2, Jingyi Niu1, Jin Huang1, Laiting Liu1, Jinrong Wang1 and Yong Zhang1*
Structure of fisetin.
Effects of fisetin on the jejunal diamine oxidase (DAO) activity in heat-stressed rats. CON, rats were orally fed with 0.5% carboxymethylcellulose sodium; HS, rats were orally fed with 0.5% carboxymethylcellulose sodium and then subjected to heat treatment; HS-FIS, rats were orally fed with 100 mg FIS /kg body weight/day and then subjected to heat treatment. Results are expressed as mean and standard error (n=8). *P<0.05 was compared with the CON group; #P<0.05 was compared with the HS group.
Effects of fisetin on the genes expression related to jejunal injury markers (A), redox status (B) and inlammation (C) in heat-stressed rats. HSP70, heat shock protein 70; I-FABP, intestinal fatty acid-binding protein; MMP3, matrix metalloproteinase 3; GPX1, glutathione peroxidase 1; Nrf2, nuclear factor, erythroid 2-like 2; SOD1, superoxide dismutase 1; Keap1, Kelch-like ECH-associated protein 1; IL6, interleukin 6; IL10, interleukin 10; TLR4, toll-like receptor 4; TNF-α, tumor necrosis factor alpha; IFN-γ, interferon γ; CON, rats were orally fed with 0.5% carboxymethylcellulose sodium; HS, rats were orally fed with 0.5% carboxymethylcellulose sodium and then subjected to heat treatment; HS-FIS, rats were orally fed with 100 mg FIS /kg body weight/day and then subjected to heat treatment. Results are expressed as mean and standard error (n=8). *P<0.05 was compared with the CON group; #P<0.05 was compared with the HS group.
Effects of fisetin on the jejunal tumor necrosis factor alpha (TNF-α) level (A) and myeloperoxidase (MPO) activity (B) in heat-stressed rats. CON, rats were orally fed with 0.5% carboxymethylcellulose sodium; HS, rats were orally fed with 0.5% carboxymethylcellulose sodium and then subjected to heat treatment; HS-FIS, rats were orally fed with 100 mg FIS /kg body weight/day and then subjected to heat treatment. Results are expressed as mean and standard error (n=6). *P<0.05 was compared with the CON group; #P<0.05 was compared with the HS group.
