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Influence of Exogenous AHLs and Quorum Quenching AiiA Protein on the Production of Cellulase Enzyme in Marine Snow Associated Bacterium, Citrobacter freundii B1

Influence of Exogenous AHLs and Quorum Quenching AiiA Protein on the Production of Cellulase Enzyme in Marine Snow Associated Bacterium, Citrobacter freundii B1

Abdul Nabi Jatt1,2*

1College of Marine Life Sciences, Ocean University of China, Qingdao (266003), China
2Institute of Microbiology, University of Sindh, Jamshoro 76080, Pakistan

*      Corresponding author: abdul.nabi@usindh.edu.pk 

Fig. 1.

Production of signaling molecules (e.g., AHLs) in sterile supernatants of C. fruendii B1. A shows the production of signaling molecules by A136 biosensor and B indicates determination of signaling molecules by CV026 biosensor. Mainly, B1 represents sterile supernatants of the bacterial strain C. freundii B1, showing AHL production, 3OC6-HSL is a synthetic AHL represents positive control and LV4 (sterile supernatants of Staphylococcus epidermis) shows negative control in well diffusion bioassay.

Fig. 2.

Sterile culture extracts of marine snow isolated bacterium C. freundii B1 (A) and the mixture of six standards (synthetic AHLs) with their retention times (B) at m/z 143 in SIM mode of GC-MS.

Fig. 3.

Enzymatic agar plate assay showing an extracellular cellulase enzyme production by C. freundii B1. Mainly, agar plate was supplemented with 1% carboxymethyl-cellulose (CMC).
 

Fig. 4.

Effect of signaling molecules (exogenous synthetic AHLs) and quorum-quenching (QQ) AiiA protein on an enzyme exoglucanase (A), endoglucanase (B) and the supernatants of the bacterium C. freundii B1. All the final results are based on means ± SD of triplicate determinations.

Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

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