In Tuberculous Cattle Transiting Early Peripartum Period Specific In Vitro PBMC Stimulation Induces an Increase of CD14 Expressing Cells, B B2 Cells, and CD25 Cells with Half IFNγ Production
María Julia Traversa1*, Mónica Saracco2, María Alejandra Colombatti Olivieri3, Fernando Paolicchi4, Edgardo Rodriguez5, Silvia Estein5, María Cristina Jorge5, William C. Davis6
1Sanidad Animal y Medicina Preventiva (SAMP), Facultad Ciencias Veterinarias (FCV), Centro de Investigación Veterinaria de Tandil (CIVETAN), Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA) CICPBA, CONICET, Pinto 399, Tandil, Buenos Aires, Argentina; 2Instituto de Investigaciones Biomédicas en Retrovirus y SIDA, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Ciudad Autónoma de Buenos Aires, Argentina; 3Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Hurlingham, Buenos Aires, Argentina; 4Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Ruta 226 Km 73.5, Balcarce, Argentina; 5SAMP, FCV, CIVETAN, UNCPBA, CICPBA, CONICET, Pinto 399, Tandil, Buenos Aires, Argentina; 6Veterinary Microbiology and Pathology Department, College of Veterinary Medicine, Washington State University, Pullmann, WA, USA.
*Correspondence | María Julia Traversa, Sanidad Animal y Medicina Preventiva (SAMP), Facultad Ciencias Veterinarias (FCV), Centro de Investigación Veterinaria de Tandil (CIVETAN), Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA) CICPBA, CONICET, Pinto 399, Tandil, Buenos Aires, Argentina; Email:
[email protected]
Figure 1:
IFN-γ production and MTT reduction to formazan of PBMC after PPDB stimuli.
IFN-γ production in mean OD ± SD(Graphic 1A) and metabolic activity of PBMC after PPDB stimuli measured through MTT stimulation index in mean percentages ± SD (Graphic 1B). PBMC were from cows PPDbEPPp , PPDbNoEPPp ▨, NoPPDbEPPp and NoPPDbNoEPPp ░.
Figure 2:
Resting (r) and stimulated (s) leukocytes subsets before and after PPDb stimuli. Resting (r) and stimulated (s) LTγδ (Graphic 2A), CD14 leukocytes (Graphic 2B), B B2 (Graphic 2C), CD25 activation marker (Graphic 2D), LT (Graphic 2E), LTCD8 (Graphic 2F), LTCD4 (Graphic 2G) and CD45Ro (Graphic 2H) are shown in mean percentages with standard deviation bars. Resting and stimulated leukocytes subsets were set with FACS before and after PPDb stimuli (20 µg/mL), respectively. Values were compared with multiple paired t test and significance was signaled with * when p<0.05
Table 3:
Individual resting (r) and stimulated (s) mean leukocytes subsets with standard deviation (SD) in PPDbEPPp, PPDbNoEPPp, NoPPDbEPPp, and NoPPDbNoEPPp cattle obtained with flow cytometry (FC) (results are presented in percentages).
