This paper describes an assessment of the Impact of weather changes and human visitation on behavior and activity level of Humboldt penguin (Spheniscus humboldti) in captivity. The study is based on activity time-budgets from a flock of over 20 birds at Fota Wildlife Park, Cork, Ireland, gathered during daytime observations over a six month period. The results show that penguins spent most of the daytime in stationary, terrestrial locomotion, preening and swimming modes of behavior, and swimming activity increased on rainy days. Peak activity took place from 16:00 to 17:00 hours, especially before and after feeding. The behavior of penguins was significantly affected by the number of visitors, and locomotion, preening, feeding and attention to visitors were positively correlated with the number of visitors. There is further interaction with visitation levels under different weather conditions. Visitors offer another positive environmental enrichment for this group of penguins and in turn, these penguins at Fota Wildlife Park are considered to have a high educational potential. Some suggestions about captive management and future research are also proposed.