Immunoregulatory Effect of Toll Like Receptor 9 Signaling Pathway in Neonatal Acute Lung Injury
Immunoregulatory Effect of Toll Like Receptor 9 Signaling Pathway in Neonatal Acute Lung Injury
Jun Mei*
ABSTRACT
The purpose of this study was to investigate the impact of toll like receptor 9 (TLR9) signaling pathway and its immunoregulatory roles in neonatal acute lung injury (NALI) , laying a theoretical basis for the clinical therapy of NALI. Forty peripheral blood mononuclear cells (PBMCs) of newborns with acute lung injury (ALI) were recruited as experimental group (EG), and another 40 PBMCs of normal newborns were selected as normal group (NG). PCR was used to detect TLR9 mRNA expression. The PBMCs were cultured by non-CpG ODN (control group, CG), CpG ODN (experimental group, EG), and blank culture (BG) for 72 h. Proliferation of PBMCs was measured with a β liquid scintillator, and the concentration of interferon (IFN)-α was determinded. It was revealed that the intensity of TLR9 mRNA expression in the PBMCs of patients with ALI was 0.76±0.06, which differed slightly versus NG (0.74±0.04) (P>0.05). The number of proliferating cells was markedly different from the CG and the BG after using TLR9 activator (P<0.05); TLR9 agonist could increase IFN-α production obviously versus CG and BG (P<0.05). It was concluded that the intensity of TLR9 mRNA expression in PBMCs of patients with ALI differed inconsiderably from that of healthy newborns. TLR9 signaling pathway was involved in immune regulation in newborns with ALI, including enhancing the proliferation ability of PBMC and increasing the production of IFN-α.
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