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Identification and Genotyping of SNPs in RKM1 and RKM4 Genes of Sordaria fimicola

Identification and Genotyping of SNPs in RKM1 and RKM4 Genes of Sordaria fimicola

Iqra Mobeen1*, Rabia Arif1*,Maimoona Ilyas1, Siu Fai Lee2 and Muhammad Saleem1

1Molecular Genetics Research Laboratory, Department of Botany, University of the Punjab, Lahore 54590, Pakistan
2Department of Biological Sciences, Macquarie University and CSIRO Black Mountain Laboratories, Building 101, Clunies Ross Street, ACT 2601, Australia

*      Corresponding author: iqramobeen905@gmail.com; phdgenetics@gmail.com

Fig. 1.

Multiple sequence alignment of RKM1 region of NFS and SFS strains of S. fimicola with respect to the Saccharomyces cerevisiae. Symbol (*) showing conserved sites, space and highlighted regions showing SNPs. Four primers specific to SNP sites are; rkm1F1, rkm1F2, rkm1F3, rkm1F4.

Fig. 2.

Multiple sequence alignment of RKM4 region of S. fimicola strains with respect to S. cerevisiae in order to observe single nucleotide polymorphism (SNP). Symbol (*) is showing fully conserved sites, space and highlighted regions showing SNPs. Four primers specific to SNP sites are; rkm4F1, rkm4F2, rkm4F3, rkm4F4.

Fig. 3.

Schematic diagram of RKM1 (A) and RKM4 (B) regions of S. fimicola showing primers specific to SNP sites to perform multiplex PCR reaction. rkm1F1, rkm1F2, rkm1F3and rkm1F4 are specific for SNPs of NFS and SFS strains, while RKM1R1 is reverse primer specific for RKM1 region.

Pakistan Journal of Zoology

February

Vol. 54, Iss. 1, Pages 1-501

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