HSPC117 Improves the Rate of Embryonic Development by Up-Regulating the Transcription of Proto-Oncogenes
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*
Heilongjiang Academy of Agriculture Science, Harbin 150086, P.R. China
* Corresponding author: mahong_ok@163.com; liudi1963@163.com
Fig. 1.
Identification of positive cell lines. A, HSPC117-transfected porcine fetal fibroblast cells line; B, HSPC117-transfected cell line confirmed using three differential size specific primer pairs.
Fig. 2.
Relative HSPC117 mRNA levels of cell clones. A, detection of HSPC117 mRNA expression in positive cell clones; B, electrophoresis of HSPC117 PCR product of cells.
Fig. 3.
Early embryonic development. A, HSPC117 transgenic embryo; B, pcDNA3.1 embryo (control); C, HSPC117 mRNA relative expression level.
Fig. 4.
Relative expression levels of c-Fos, c-Jun, Raf-1, c-Myc and control mRNA in HSPC117-transfected reconstructed blastocysts.