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Genome Survey and Large-scale Isolation of Microsatellites through Illumina Sequencing from the Red Swamp Crayfish Procambarus clarkii

Genome Survey and Large-scale Isolation of Microsatellites through Illumina Sequencing from the Red Swamp Crayfish Procambarus clarkii

Xiao Zhang1, Guoqing Shen1, Yang Wang1, Pengdan Huang1,2, Yan Shui2, Zenghong Xu2 and Huaishun Shen1, 2*

1Wuxi Fisheries College, Nanjing Agricultural University, Nanjing, 210095, PR China
2Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Road, Wuxi 214081, PR China
 
*      Corresponding author: shenhuaishun@ffrc.cn

Fig. 1.

Results of a genomic survey using Illumina sequencing. (A), A K-mer (K=17) analysis to estimate the genome size of Procambarus clarkii. The x-axis is the depth (X); the Y-axis is the proportion that represents the frequency at that depth divided by the total frequency of all depths. (B), GC content and average sequencing depth. The X-axis represents the GC content, and the Y-axis represents the average depth. The red region represents the relatively dense part of the points in the scatter plot.

Fig. 2.

Distribution of SSR motifs in Procambarus clarkii. The Y-axis represents motif types and the X-axis represents the count of motifs in whole genome of Procambarus clarkii.

Fig. 3.

The amplification band of scaffold1946501 loci for 39 individuals of Procambrus clarkii. The M is Marker; 150 is 150 bp, and 100 is 100 bp.

Pakistan Journal of Zoology

February

Vol. 55, Iss. 1, Pages 1-500

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