Genetic Relatedness among the Indigenous Isolates of Trichoderma harzianum, using RAPD and their Nematocidal Capabilities against Meloidogyne javanica
Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1
1Department Microbiology, Kohat University of Science and Technology Kohat, Pakistan; 2Department Plant Pathology, The University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan; 3Departmnt Weed Science, The University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan; 4Department Biotechnology and GE, Kohat University of Science and Technology Kohat, Pakistan.
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Figure 1
PCR profile of T. harzianum isolates using RAPD primers: A. GLD-17, B.GL-G 20, C. GL-D08.
Figure 2
Dendrogram (with three RAPD primers) showing genetic similarities among 15 isolates of T. harzianum using the unweighted pair group method with arithmetical average (UPGMA) of Genetyx software, (version 7.0). RAPD clusters are indicated in the right.