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Genetic Relatedness among the Indigenous Isolates of Trichoderma harzianum, using RAPD and their Nematocidal Capabilities against Meloidogyne javanica

Genetic Relatedness among the Indigenous Isolates of Trichoderma harzianum, using RAPD and their Nematocidal Capabilities against Meloidogyne javanica

Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1 

1Department Microbiology, Kohat University of Science and Technology Kohat, Pakistan; 2Department Plant Pathology, The University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan; 3Departmnt Weed Science, The University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan; 4Department Biotechnology and GE, Kohat University of Science and Technology Kohat, Pakistan.

baharkk75@yahoo.com  

Figure 1

PCR profile of T. harzianum isolates using RAPD primers: A. GLD-17, B.GL-G 20, C. GL-D08. 

Figure 2

Dendrogram (with three RAPD primers) showing genetic similarities among 15 isolates of T. harzianum using the unweighted pair group method with arithmetical average (UPGMA) of Genetyx software, (version 7.0). RAPD clusters are indicated in the right. 

Sarhad Journal of Agriculture

June

Sarhad Journal of Agriculture, Vol.40, Iss. 2, Pages 263-679

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