Genetic Diversity of Mycobacterium tuberculosis Complex Prevailing In Khyber Pakhtunkhwa, Pakistan
Genetic Diversity of Mycobacterium tuberculosis Complex Prevailing In Khyber Pakhtunkhwa, Pakistan
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
Comparative evaluation of sensitivity of fluorescence microscopy and GeneXpert assay.
Immunochromatographic test for samples 2 to 5 to differentiate MTBC from other mycobacterial species.
Dendrogram showing genetic relatedness among MTBC strains.
Agarose gel electrophoresis of PCR products. Lane 1, DNA Marker; Lane 2, H37Rv; Lane 3, M. bovis; Lane 4 to 15, MTBC clinical strains with visible amplification at 439.
A, Agarose Gel Electrophoresis showing MTBC isolates after digestion with BstEII restriction enzyme. Lane 1, DNA marker; Lane 2, positive control H37Rv; Lane 3, M. bovis; Lane 4 to 16, MTBC clinical strains. B, Agarose Gel Electrophoresis showing MTBC isolates after digestion with HaeIII restriction enzyme. Lane 1, DNA marker; Lane 2, H37Rv; Lane 3, M. bovis; Lane 4 to 10, MTBC clinical strains.