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Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System

Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5

1School of Life Sciences and Technology, Bandung Institute of Technology, Jl. Ganesa 10, Bandung 40132, West Java-Indonesia; 2Department of Agriculture Science, Postgraduate Program, University of Muhammadiyah Malang, Jl. Raya Tlogomas No. 246, Malang, 65145, East Java, Indonesia; 3Department of Biotechnology Engineering, International Islamic University Malaysia, Jl. Gombak, 53100, Selangor, Malaysia; 4Department of Agrotechnology, Merdeka University of Madiun, Jl. Serayu No.79, Madiun 63133, East Java, Indonesia; 5Department of Water Management, Estonian University of Life Sciences, Tartu, Estonia; Friedrich Reinhold Kreutzwaldi 1a, 51014 Tartu, Estonia.

 
*Correspondence | Rizkita Rachmi Esyanti, School of Life Sciences and Technology, Bandung Institute of Technology, Jl. Ganesa 10, Bandung 40132, West Java-Indonesia; Email: rizkita@sith.itb.ac.id

Figure 1:

Shoots before and after cultivation in RITA® bioreactor: (A) Day 0; (B) Days 21 for RITA® Control (No Exposed with High Far-Red LED Light); and (C) Days 21 for RITA® LED (Exposed with High Far- Red Light LED for 1 hour during night cycle).

Figure 2:

Gene expression profiles related to steviol glycosides synthesis of S. rebaudiana shoot cultivated in RITA® high far-red LED bioreactors compared with control (as a baseline with 1 relative mRNA level); (**: significantly different by student’s test with p-value < 0.01).

Sarhad Journal of Agriculture

March

Sarhad Journal of Agriculture, Vol.40, Iss. 1, Pages 01-262

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