First Report of Leishmania infantum in a Captive Panther from Pakistan
First Report of Leishmania infantum in a Captive Panther from Pakistan
Wafa Akram1, Shahan Azeem2, Shafqat Shabir1, Haroon Akbar1*, Warda Gill3 and Muhammad Imran Rashid1*
(a) Microscopic image captured with an i-Phone-7 from stained blood smear of tiger blood sample examined at 1000x magnification in an Olympus CX-21 microscope. The inset contains images of two monocytes that were edited with photos editor of windows, with a Zeke Filter on it, for a clearer view of the multiple intracellular Leishmanial amastigotes (Thick yellow arrow pointed to one of those) that actually helped in diagnosis. (b) An image of agarose gel indicating a band at the size expected for L. infantum detection in a tiger blood. C+ and C- represent positive and negative controls, respectively.
Phylogenetic analyses of L. infantum from a tiger in Pakistan (a) with Leishmania sequences from animals and (b) humans.
