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Eye Color Frequency and IrisPlex Validation in Pakhtun Population Living in Shangla Valley, Pakistan

Eye Color Frequency and IrisPlex Validation in Pakhtun Population Living in Shangla Valley, Pakistan

Murad Ali Rahat1*, Muhammad Israr2, Hakim Khan1, Ishtiaq Hassan1, Mohammad Islam1 and Muzafar Shah3

1Department of Biotechnology and Genetic Engineering, Hazara University Mansehra, Pakistan
2Department of Forensic Sciences, University of Swat, Swat, Pakistan
3Centre for Animal Sciences and Fisheries, University of Swat, Swat, Pakistan
 
* Corresponding author: Rahatmurad32@yahoo.com

Fig. 1.

Showing singleplex (A) and Multiplex-PCR amplification result (B) of six SNPs. A, Lane 1, 50 bp DNA ladder; lanes 2, 6, 10 and 14 IRF4, 3, 7, 11; Lane 15, SLC45A2; Lane 4, 8, 12 and 16 SLC24A4; Lanes 5, 9, 13 and 17 OCA2, 18, 19, 20; Lane 21 showed TYR and Lines 22, 23, 24 and 25, HERC2 SNP. In B, the 4% gel figure showed Line 1 50bp DNA marker; lines 2-5 showed the combined result of OCA2, TYR and IRF4, while lines number 6-9 showed the combined result of HERC2, SLC24A4 and SLC45A2.

Fig. 2.

Genotypes of six SNPs in the study population.

Pakistan Journal of Zoology

December

Vol. 54, Iss. 6, Pages 2501-3000

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