Expression Profiling of microRNAs hsa-222-3p, hsa-let-7b-5p, hsa-let-7f-5p and their Putative Targets HMGA1 and CDKN1B Genes in Canine Mammary Tumor
Expression Profiling of microRNAs hsa-222-3p, hsa-let-7b-5p, hsa-let-7f-5p and their Putative Targets HMGA1 and CDKN1B Genes in Canine Mammary Tumor
Hafiz Muhammad Farooq Yaqub1, Sehrish Firyal1, Ali Raza Awan1, Muhammad Tayyab1, Rashid Saif2, Muti ur Rehman3, Muhammad Wasim1*
Histopathological analysis of dog mammary tumor. (a) Representative dog mammary tumors showed varied population of cells with enormous nucleus and prominent mitotic characteristic. (b) Numerous key regions indicating the presence of fibroblasts and collagen fibers.
Comparison of hsa-mir-222-3p, hsa-let-7b-5p and hsa-let-7f-5p expression (fold change) in dog mammary tumor samples (DMT) using RT-qPCR.
Expression data of miRNAs (fold change), in dog tumor (DMT) and normal mammary tissue (DNS) samples, was analyzed using GraphPad Prism software. (a) hsa-mir-222-3p, (b) hsa-let-7b-5p and (c) hsa-let-7f-5p.
Comparison of hsa-mir-222-3p, hsa-let-7b-5p and hsa-let-7f-5p expression in dog mammary tumor samples (DMT) using using GraphPad Prism software.
Expression data of target genes, in dog tumor (DMT) and normal mammary tissue (DNS) samples, was analyzed using GraphPad Prism software. (A) Fold change of HMGA1 gene in dog mammary tumor. (B) Fold change of CDKN18 gene in dog mammary tumor. (C) Comparison of HGMA 1 and CDKN 1B genes expression in dog mammary tumor samples (DMT) using using GraphPad Prism software.
Expression level of hsa-mir-222-3p (A, B), hsa-let-7b-5p (C, D) and hsa-let-7f-5p (E, F) miRNA in canine mammary tumor was measured using RT-qPCR. C, E data from three technically replicated measurements were averaged and normalized to the internal RNU6B control. Log 2-fold change values were calculated for 10 dog mammary tumor samples (DMT). D, F, Relative expression of for all the three hsa-mir-222-3p miRNA using RNU6B normalized data of cancer (DMT) vs normal tissues (DNS) to calculate ∆Ct.
