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Evaluation of Methicillin Resistance in Field Isolates of Staphylococcus aureus: An Emerging Issue of Indigenous Bovine Breeds

Evaluation of Methicillin Resistance in Field Isolates of Staphylococcus aureus: An Emerging Issue of Indigenous Bovine Breeds

Nauman Zaheer Ghumman, Muhammad Ijaz*, Arslan Ahmed, Muhammad Umar Javed, Iqra Muzammil and Ahmed Raza

Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore

 
* Corresponding author: mijaz@uvas.edu.pk

Fig. 1.

QGIS map showing the study area.

Fig. 2.

Phylogenetic Tree showing comparison of the study isolates of MRSA with reported isolates.

Fig. 3.

Manually labeled SDS-PAGE comparison results of MRSA and MSSA with a determined molecular weight of each band. (R1 to R5) shows MRSA isolates, while, (S1 to S4) shows the MSSA. The white arrow shows the presence of 78kDa band of PBP2a protein of MRSA and absent in MSSA. (Yellow arrows) Shows the difference in protein bands present in MRSA, (Black arrows) shows the difference in protein bands present in MSSA.

Fig. 4.
A standard Curve obtained by plotting a scatter graph between Rf values and log of known molecular weight of protein marker. The X-axis shows the Rf values ranging from 0 to 0.9. Y-axis shows the log of molecular weight ranging from 4.2 to 5.2. The regression formula (y = -0.9692x + 5.0902) obtained was used to calculate the molecular weight of all the unknown proteins separated on the gel. Where Y= Rf value of that specific protein band and X= Molecular weight of unknown protein band.

Pakistan Journal of Zoology

February

Vol. 55, Iss. 1, Pages 1-500

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