Enhanced Activities of Metabolic Enzymes Associated with Insecticide Resistance in Red Flour Beetle (Tribolium castaneum Herbst)
Muhammad Asam Riaz1, Javeria Choudhary1, Muhammad Zeeshan Majeed1*, Muhammad Afzal1, Sohail Ahmed2 and Muhammad Sajjad Khalil1
1Department of Entomology, College of Agriculture, University of Sargodha, Sargodha 40100, Pakistan; 2Department of Entomology, University of Agriculture, Faisalabad 38000, Pakistan.
zeeshan.majeed@uos.edu.pk
Figure 1
Cellulase and amylases activities in different strains of T. castaneum adults.
Figure 2
Line-weaver Burk plots for determining the kinetic constants (Vmax and Km) of cellulases and amylases in different strains of T. castaneum adult. Crude enzyme extract (50 µL) was incubated with 10-40 and 20-50 g L-1 of starch for cellulases and amylases respectively.