Research Article

Efficacy of Soil Applied Phosphorus and Foliar Zinc Application on Different Growth Stages to Improve Productivity of Maize (Zea mays)

Sajid Ali1*, Shahen Shah2, Muhammad Amin2, Asad Ali Khan2, Dawood Ahmad4, Ikram Ullah5, Faiq Ahmad2, Sajjad6 and Sikandar Azam1

1Livestock Research and Dairy Development Department, Government of Khyber Pakhtunkhwa, Peshawar, Pakistan; 2Department of Agronomy, Faculty of Crop Production Sciences, The University of Agriculture, Peshawar, Pakistan; 3Department of Environmental Sciences, Shaheed Benazir Bhutto University, Sheringal, Upper Dir, Khyber Pakhtunkhwa, Pakistan; 4Institute of Biotechnology & Genetic Engineering (IBGE), The University of Agriculture, Peshawar, Pakistan; 5Department of Agriculture, Bacha Khan University, Charsadda Khyber Pakhtunkhwa, Pakistan; 6Senior Scientific Officer, Crop Science Institute, National Agricultural Research Centre, Islamabad, Pakistan.

Received | March 03, 2022; Accepted | March 23, 2022; Published | July 14, 2022

*Correspondence | Sajid Ali, Livestock Research and Dairy Development Department, Government of Khyber Pakhtunkhwa, Peshawar, Pakistan; Email: [email protected]

Citation | Ali, S, S. Shah, M. Amin, A.A. Khan, D. Ahmad, I. Ullah, F. Ahmad, S. Khan and S. Azam. 2022. Efficacy of soil applied phosphorus and foliar zinc application on different growth stages to improve productivity of maize (Zea mays). Sarhad Journal of Agriculture, 38(3): 912-917.

DOI | https://dx.doi.org/10.17582/journal.sja/2022/38.3.912.917

Keywords | Application time, Maize, Phosphorus, Yield components, Zinc

Copyright: 2022 by the authors. Licensee ResearchersLinks Ltd, England, UK.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Introduction

Fertilizers play an important role in crop production and also enhanced quality of crop (Amjadian et al., 2021). Balanced fertilizers are very important because it’s improved and enhances plant development and also increases yield (Hitha et al., 2021). Among the essential nutrients, phosphorus (P) is the second essential nutrient. Phosphorus (P) is important nutrient in the cropping system of agriculture (Roberts and Johnston, 2015; Guignard et al., 2017; Khan et al., 2018). It plays a role in crop development and in its metabolism (Yadav et al., 2017). Phosphorus is the part of nucleic acids, structural proteins and of enzymes (Yao et al., 2018). After nitrogen, phosphorus increases yield of crop (Adeyemi et al., 2020). Most of the cereal and vegetable crops require phosphorus in large quantity (Ketterings et al., 2020). It has two different forms in soil i.e. H2PO4-1 and HPO4-2 and plant takes it in these two forms. The absorption of HPO4-2is very rapid and faster as compare to H2PO4-1 (Alamnie and Misganew, 2021). Application of phosphorus give strength to straw of cereal crop and as a result crop lodging decreases. It also increases resistance to many diseases. It plays a crucial role in cell division, nucleus formation, helps in DNA and RNA formation (Kolodiazhnyiet al., 2021). Deficiency of phosphorus limits development and growth of plant (Kewei et al., 2014). Phosphorus decreases yield in maize crop when applied in low amount (Cengiz et al., 2020).

Micro-nutrients though are as important as macro-nutrients (Jake et al., 2022). However, as compared to macronutrients, micronutrients are required in very small amount. Generally, in most cases, both the plants and soils have very small amounts of micro-nutrients (Chakraborty et al., 2021). Micro-nutrients play a key role in plant growth and its development, improve crop phenology and also involve in various physiological processes (Hassan et al., 2019). Similarly, in case of micro-nutrients, Zinc (Zn) is an important micro-nutrient and plays a key role in plants (Natasha et al., 2022). Zn is necessary for biosynthesis of chlorophyll (Ali et al., 2021). In general, Zn has major role in activating enzyme, proteins synthesis, revival and oxidation reactions and carbohydrates metabolism (Ali et al., 2021). The deficiency of Zn may lead to photosynthesis decline and destruction of RNA, decreased protein synthesis, carbohydrates solution and thus affecting performance and quality of crop (Kadyampakeni and Chinyukwi, 2021). Foliar application of both Zn and Fe also enhances the phenological development and yield component of wheat crop (Ali et al., 2021). Application of Zn enhances height of maize crop when applied at split doses. Foliar Zn also significantly enhances size of maize leaf (Raheela et al., 2021).

Materials and Methods

Field experiment

The experiment entitled “efficacy of soil applied phosphorus and foliar zinc application on different growth stages to improve productivity of maize (Zea mays) was performed at Fodder and Forage crops section Harichand, Charsadda, Khyber Pakthunkhwa; Pakistan. The field experiment was conducted on 7 July 2021. The experiment comprised of the following factors along with their respective levels. Two levels of P (90 and 120 kg ha-1) were applied at the time of sowing and three levels of Zn (0 i.e., water spray only, 2.5 and 5 kg ha-1) were applied at three growth stages i.e., full at vegetative stage (V6), full at reproductive stage (R2) and½ at V6 and ½ at R2 stage along with an overall control were used in the experiment. The experiment was conducted in randomized complete block design with three replications. Treatment combinations of all the three factors along with a control were randomly allotted to the experimental plots in each block. Plot size was 4.9 × 4.0 m. Jalal variety was used for sowing. Planting was made on flat beds in rows spaced 0.70m. For application of Zn, 10% solution of ZnSO4.H2O was prepared. Keeping in view the treatments and volume to wet the subplot area completely, the solution was further diluted with water. Control plots were sprayed with equivalent quantity of water. First irrigation was given on 19 July 2021after sowing and subsequent irrigation was adjusted according to the need of crop. For all the treatments, other agronomic practices were kept uniform.

Locality of the experimental site

The experimental field is placed at 34.020 N, 71.470 E and 331 m above the level of sea. The mean annual rainfall of the location is 360 mm. The mean maximum summer temperature is 40 oC and the mean minimum is 25 oC while in winter minimum mean temperature is 4 oC and mean maximum temperature is 18.34 oC.

Soil sample collection and laboratory analysis

The soil characteristics as evaluated in laboratory and classified the soil as texture silty clay loam having 51.3% silt, 40% clay and 8.7% sand, 14.4% CaCO3, 8.08 pH and having low organic matter of 0.58% g kg-1. Similarly, the mineral contents of soil were i.e. 0.21 ppm zinc, 66.38 ppm iron, 2.86 ppm nickel, 1.07 ppm lead, .085 ppm chromium and 0.937 ppm was copper.

Statistical analysis

The data noticed on different parameters was analyzed statistically according to the procedure relevant to randomized complete block design as described by little and Hills (1978). Least significant difference (LSD) test were used for mean separation when the F test was significant.

Results and Discussion

Ear length (cm)

Data of ear length showed in Table 1. Maximum ear length (17.8 cm) was noticed when 120 kg P ha-1was applied while minimum (16.8 cm) was noticed when 90 kg P ha-1was applied. The reason for increased of ear length of maize might be that phosphorus could translocate assimilation to ears has accrued and have been resulted in to increased ear length (Juan et al., 2021). Similarly, in case of Zn, maximum ear length (18.4 cm) was observed when 5 kg Zn ha-1was applied while minimum (16.1 cm) was noticed with water spray only. Ear length and weight increased with higher rate of Zn (Hisham et al., 2021). In case of Zn application timing, maximum ear length (18.2 cm) was recorded when Zn was applied all at AT3 stage while minimum (16.5 cm) was noticed when applied all at AT1 stage.

Ear weight (g)

Data concerning ear weight are accessible in Table 2. Maximum ear weight (115.8 g) was noticed when 120 kg P ha-1was applied while minimum (109.4 g) was observed when 90 kg P ha-1was applied. Ear weight of maize crop increased with higher rate of phosphorus because phosphorus increases the amount of endosperm in grain (Perkins et al., 2021). Similar in case of Zn, maximum ear weight (129.6 g) was noticed when 5 kg Zn ha-1was applied while minimum (113.4 g) was noticed with water spray only. Increase of ear weight is due to the heavier grain weight, because zinc provided adequate amount of carbohydrates to source. Therefore, ear weight increases with the application of Zn (Liu et al., 2020). In case of Zn application timing, maximum ear weight (127.4g) was shown when all Zn was applied at AT3 stage while minimum (111.4 g) was noticed with all at AT2 stage. In such away in case of P x AT interaction, the maximum ear weight (124.1 g) was noticed in plots when 120 kg P ha-1 and Zn applied half at V6 and half at R2 stage while minimum (97.9 g) was noticed in plot that received 90 kg P ha-1 and all Zn at AT2 stage.

 

Table 1: Ear length (cm) of maize as affected by phosphorus, foliar Zn and its application timing.

Phosphorus (kg ha-1)	Zn (kg ha-1) application	Growth stages			Mean
		V6	R2	50% at V6 + 50% at R2
90	0	13.3	17.0	16.7	15.7
	2.5	16.3	17.1	17.2	16.9
	5.0	17.5	17.3	18.8	17.9
120	0	15.8	16.7	17.2	16.6
	2.5	17.0	17.4	19.0	17.8
	5.0	19.0	17.3	20.3	18.9
-	0	14.6	16.9	16.9	16.1 c
-	2.5	16.7	17.2	18.1	17.3 b
-	5.0	18.2	17.3	19.6	18.4 a
90	-	15.7	17.1	17.6	16.8 b
120	-	17.3	17.1	18.8	17.8 a
Mean		16.5 b	17.1 b	18.2 a
Control					13.0 b
Rest					17.3 a

 

Table 2: Ear weight (g) of maize as affected by phosphorus, foliar Zn and its application timing.

Phosphorus (kg ha-1)	Zn (kg ha-1) Application	Growth stages			Mean
		V6	R2	50% at V6 + 50% at R2
90	0	93.0	101.0	117.3	103.8
	2.5	110.3	96.7	110.3	105.8
	5.0	141.3	96.0	118.3	118.6
120	0	107.3	118.0	143.7	123.0
	2.5	111.3	129.0	143.3	127.9
	5.0	138.0	141.7	142.0	140.6
-	0	100.2	109.5	130.5	113.4 b
-	2.5	110.8	112.8	126.8	116.8 b
-	5.0	139.7	118.8	130.2	129.6 a
90	-	114.9	97.9	115.3	109.4 b
120	-	119.7	103.6	124.1	115.8 a
Mean		117.0 ab	111.4 b	127.4 a
Control					94.3 b
Rest					119.9 a

V6: Vegetative stage leaf 6th; R2: reproductive stage.

 

Grains ear-1

Data concerning grains ear-1 are obtainable in Table 3. In case of P, maximum grains ear-1 (390) was shown when 120 kg P ha-1was applied while minimum (369) grains ear-1 was noticed when 90 kg P ha-1was applied. Phosphorus is responsible for the growth of root which directly affects the overall plant performance (Bing et al., 2022). It has also reported that grains per ear also increase with phosphorus fertilization (El-Sobky et al., 2021). Similarly, in case of Zn, maximum grains ear-1 (407) was noticed when 5 kg Zn ha-1 was applied while minimum (353) was shown with water spray only. This is because that Zn is essential for pollen grain development and pollen viability; as a result, number of grains increases in ear (Sonal et al., 2022). In case of Zn application timing, maximum (391) was noticed when Zn was applied half at V6 and half at R2 stage while minimum (372) was noticed when all applied at AT2 stage.

 

Table 3: Grains ear-1 of maize as affected by phosphorus, foliar Zn and its application timing.

Phosphorus (kg ha-1)	Zn (kg ha-1) application	Growth stages			Mean
		V6	R2	50% at V6 + 50% at R2
90	0	313.3	343.0	386.7	347.7
	2.5	356.7	351.3	390.0	366.0
	5.0	416.7	381.3	382.7	393.6
120	0	360.0	356.7	359.7	358.8
	2.5	386.7	388.7	400.7	392.0
	5.0	414.7	416.7	430.3	420.6
-	0	336.7	349.8	373.2	353.2 c
-	2.5	371.7	370.0	395.3	379.0 b
-	5.0	415.7	399.0	406.5	407.1 a
90	-	362.2	358.6	386.4	369.1 b
120	-	387.1	387.3	396.9	390.4 a
Mean		374.7 b	372.9 b	391.7 a
Control					341.0 b
Rest					379.8 a

V6: Vegetative stage leaf 6th; R2: reproductive stage.

 

Thousand grains weight (g)

Data regarding thousand grains weight are obtainable in Table 4. Statistical analysis of the data show that maximum thousand grains weight (257 g) was seen at 120 kg Pha-1and minimum (233.7 g) thousand grains weight were seen at 90 kg p ha-1 application. Heaviest grain weight with higher P level probably may be due to higher P translocation in to the fruiting area which results in highest grain weight. Increasing in P levels increased grain weight (Hamza et al., 2022). Similarly, in case of Zn, highest (258.3 g) thousands grain weight was noticed when 5 kg Zn ha-1was applied while minimum (220.5 g) was noticed with water spray only. When the supply of carbohydrates to grain is high then the filling of grain will be high and as a result weight of kernels will be more (Wenxin et al., 2017). In case of Zn using timing, maximum thousands grain weight (255.5 g) was shown when Zn was applied half at V6 and half at R2 stage while minimum (228.6 g) was noticed when applied all at V6 stage.

 

Table 4: Thousand grains weight (g) of maize as affected by phosphorus, foliar Zn and its application timing.

Phosphorus (kg ha-1)	Zn (kg ha-1) application	Growth stages			Mean
		V6	R2	50% at V6 + 50% at R2
90	0	195	208	203	202
	2.5	200	220	253	224
	5.0	240	233	260	244
120	0	206	250	260	238
	2.5	250	266	263	260
	5.0	280	243	293	272
-	0	200	229	231	220 b
-	2.5	225	243	258	242 a
-	5.0	260	238	276	258 a
90	-	211	220	238	223 b
120	-	245	253	272	257 a
Mean		228 b	236 b	255 a
Control					206 b
Rest					240 a

V6: Vegetative stage leaf 6th; R2: reproductive stage.

 

Conclusions and Recommendations

It is concluded from the experiment that application of 120 kg P ha-1 significantly improved ear length, ear weight, grains per ear and thousand grain weight. Similarly, foliar application of 5 kg Zn ha-1 improved ear length, ear weight, grains per ear and thousand grain weight of cob. In case of application timing of zinc, when applied ½ at vegetative and ½ reproductive stage will improve productivity of maize crop. Therefore,120 kg P ha-1, 5 kg Zn ha-1 along with its application timing ½ at vegetative and ½ reproductive stage is recommended for the agro climatic conditions of Peshawar.

Novelty Statement

This study conducted to find out the impact of phosphorus, Zinc and its application stages to improve maize yield.

Author’s Contribution

Sajid Ali: Carried out research and drafted the manuscript.

Shahen Shah: Provided technical guidelines

Muhammad Amin: Helped in data analysis

Asad Ali Khan: Helped in editing and draft improvement

Dawood Ahmad and Ikram Ullah: Helped results and discussion interpretation

Faiq Ahmad and Sajjad Khan: Helped in data collection

Sikandar Azam: Helped in format setting

Conflict of interest

The authors have declared no conflict of interest.

References

Adeyemi, O., R. Afshar, E. Jahanzad, M.L. Battaglia, Y. Luo, and A. Sadeghpour. 2020. Effect of wheat cover crop and split nitrogen application on corn yield and nitrogen use efficiency. Agronomy, 10: 1081. https://doi.org/10.3390/agronomy10081081

Alamnie, A. and Misganew. 2021. Effects of organic amendments on phosphorus sorption characteristics of nitisols of Pawe district, Northwestern Ethiopia. Diss. Bahir Dar University, 2021.

Ali, S., S. Shah and M. Arif. 2021. Agronomic biofortification with zinc and iron for the improvement of wheat phenology and yield. Sarhad J. of Agric., 37(3): 901-914. https://doi.org/10.17582/journal.sja/2021/37.3.901.914

Amjadian, Elham, A. Zeinodini, and H. Dogan. 2021. Effect of fertilizer management systems on growth and balance of nutrients in wheat cultivation. Cent. Asian J. Plant Sci. Innov., 1.2(2021): 56-69.

Bing, M., Y. Wang, T.H. Zhao, Q. Zhao, Y. San, and S.S. Xiao. 2022. Response of carbon, nitrogen and phosphorus concentration and stoichiometry of plants and soils during a soybean growth season to O3 stress and straw return in Northeast China. Sci. Total Environ., pp. 153573. https://doi.org/10.1016/j.scitotenv.2022.153573

Cengiz, K., M. Senbayram, N.A. Akram, M. Ashraf, M.N. Alyemeni, and P. Ahmad. 2020. Sulfur-enriched leonardite and humic acid soil amendments enhance tolerance to drought and phosphorus deficiency stress in maize (Zea mays L.). Sci. Rep., 10(1): 1–13. https://doi.org/10.1038/s41598-020-62669-6

Chakraborty, M., D. Chakraborty, P. Mondal, and P.R. Paul. 2021. Micronutrient deficiency stress in soils of India: Tackling it to alleviate hidden hunger. In soil science: Fundamentals to recent advances, pp. 801-821. https://doi.org/10.1007/978-981-16-0917-6_40

El-Sobky, E.S.E., and A.I. Abdo. 2021. Efficacy of using biochar, phosphorous and nitrogen fertilizers for improving maize yield and nitrogen use efficiencies under alkali clay soil. J. Plant Nutr., 44(4): 467-485. https://doi.org/10.1080/01904167.2020.1845369

Guignard, M.S., A. Leitch, R. Acquisti, C., Eizaguirre, J.J. Elser, and D.O. Hessen. 2017. Impacts of nitrogen and phosphorus: From genomes to natural ecosystems and agriculture. Front. Ecol. Evol., 5: 70. https://doi.org/10.3389/fevo.2017.00070

Hamza, Z.K., A.S. Hathout, G. Ostroff, E. Soto, B.A. Sabry, M.A. ashash, and S.E. Aly. 2022. Assessment of the protective effect of yeast cell wall β glucan encapsulating humic acid nanoparticles as an aflatoxin B1 adsorbent in vivo. J. Biochem. Mol. Toxicol., 36(1): 22941. https://doi.org/10.1002/jbt.22941

Hassan, U., L. Mahmood, R. Tassaduq, C. Iqbal, A. Arshad, M. Abrar, M.M. Abrar, M.H. Rahman, M.A. Noor, A. Sher and S. Fahad. 2021. Linking plants functioning to adaptive responses under heat stress conditions: A mechanistic review. J. Plant Growth Regul., pp. 1-18.

Hisham, A.A., H.Y. Ching, M.M. Rahman, K. Mat, and A.K. Zulhisyam. 2021. Effects of zinc on the growth and yield of maize (Zea mays L.) cultivated in a tropical acid soil using different application techniques. Earth Environ. Sci., 756(1): 012056. https://doi.org/10.1088/1755-1315/756/1/012056

Hitha, S., V. Chandran and L. Mathew. 2021. Organic fertilizers as a route to controlled release of nutrients. Controlled release fertilizers for sustainable agriculture. Acad. Press, 2021: 231-245. https://doi.org/10.1016/B978-0-12-819555-0.00013-3

Jake, C., I. Ortiz-Monasterio, D. Guerena, D.B. Lobell. 2022. Evaluating maize yield response to fertilizer and soil in Mexico using ground and satellite approaches. Field Crops Res., 276: 108393. https://doi.org/10.1016/j.fcr.2021.108393

Juan, H., B. Ren, S. Dong, P. Liu, B. Zhao and J. Zhang. 2021. Poor development of spike differentiation triggered by lower photosynthesis and carbon partitioning reduces summer maize yield after waterlogging. Crop J., 2021.

Kadyampakeni, D.M., and T. Chinyukwi. 2021. Are macronutrients and micronutrients therapeutic for restoring performance of trees affected by citrus greening? A discussion of current practices and future research opportunities. J. Plant Nutr., 44(19): 2949-2969. https://doi.org/10.1080/01904167.2021.1927079

Ketterings, Q., and K. Czymmek. 2020. Removal of phosphorus by field crops, agronomy fact sheet series; fact sheet #28; Nutrient Management Spear Program, Cornell University: Ithaca, NY, USA., (accessed on 25 July 2020).

Kewei, Z., H. Liu, P. Tao and H. Chen. 2014. Comparative proteomic analyses provide new insights into low phosphorus stress responses in maize leaves. PLoS One, 9(5): 98215. https://doi.org/10.1371/journal.pone.0098215

Khan, A., G. Lu, M. Ayaz, H. Zhang, R. Wang and F. Lv. 2018. Phosphorus efficiency, soil phosphorus dynamics and critical phosphorus level under long-term fertilization for single and double cropping systems. Agric. Ecosyst. Environ., 256: 1–11. https://doi.org/10.1016/j.agee.2018.01.006

Kolodiazhnyi and I. Oleg. 2021. Phosphorus compounds of natural origin: Prebiotic, Stereochemistry, Application. Symmetry, 13(5): 889. https://doi.org/10.3390/sym13050889

Liu, D.Y., W. Zhang, Y.M. Liu, X.P. Chen and C.Q. Zou. 2020. Soil application of zinc fertilizer increases maize yield by enhancing the kernel number and kernel weight of inferior grains. Front. Plant Sci., 11: 188. https://doi.org/10.3389/fpls.2020.00188

Natasha, N., M. Shahid, I. Bibi, J. Iqbal, S. Khalid, B. Murtaza, H.F. Bakhat, A.B. Farooq, M. Amjad, H.M. Hammad and N.K. Niazi. 2022. Zinc in soil plant human system: A data-analysis review. Sci. Total Environ., 808: 152024. https://doi.org/10.1016/j.scitotenv.2021.152024

Nazia, H., S. Irshad, M.S. Saddiq, S. Bashir, S. Khan, M.A. Wahid, R.R. Khan, and M. Yousra. 2019. Potential of zinc seed treatment in improving stand establishment, phenology, yield and grain biofortification of wheat. J. Plant Nutr., 42(14): 1676-1692. https://doi.org/10.1080/01904167.2019.1630429

Perkins, A.C., and J.P. Lynch. 2021. Increased seminal root number associated with domestication improves nitrogen and phosphorus acquisition in maize seedlings. Ann. Bot., 128(4): 453-468. https://doi.org/10.1093/aob/mcab074

Raheela, R., M. Asif, I. Cakmak and L. Ozturk. 2021. Differences in uptake and translocation of foliar-applied Zn in maize and wheat. Plant Soil, 462(1): 235-244. https://doi.org/10.1007/s11104-021-04867-3

Roberts, T.L., and A.E. Johnston. 2015. Phosphorus use efficiency and management in agriculture. Res. Cons. Rec., 105: 275–281. https://doi.org/10.1016/j.resconrec.2015.09.013

Sonal, K., G. Verma, and S.S. Dhaliwal. 2021. Zinc phasing and fertilization effects on soil properties and some agromorphological parameters in maize wheat cropping system. Commun. Soil Sci. Plant Anal., 4: 1-10.

Wenxin, L., Z. Zhang, X. Wen, Y. Liao, and Y. Liu. 2017. Effect of non-structural carbohydrate accumulation in the stem pre-anthesis on grain filling of wheat inferior grain. Field Crops Res., 211: 66-76. https://doi.org/10.1016/j.fcr.2017.06.016

Yadav, H., R. Fatima, A. Sharma and S. Mathur. 2017. Enhancement of applicability of rock phosphate in alkaline soils by organic compost. Appl. Soil Ecol., 113: 80–85. https://doi.org/10.1016/j.apsoil.2017.02.004

Yao, Q., Z. Li, Y. Song, S.J. Wright, X. Guo, S.G. Tringe and C. Pan. 2018. Community proteogenomics reveals the systemic impact of phosphorus availability on microbial functions in tropical soil. Nat. Ecol. Evol., 2(3): 499-509. https://doi.org/10.1038/s41559-017-0463-5