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Effects of Newcastle Disease Infection on the mRNA Expression of Inflammatory Biomarkers in Naturally Infected Chicks

Effects of Newcastle Disease Infection on the mRNA Expression of Inflammatory Biomarkers in Naturally Infected Chicks

Hakeem Jawad Kadhim1, Abbas Kamil Shlaga1*, Safaa Hussein Ali2 

1Department of Microbiology, College of Veterinary medicine and Surgery, Shatrah University, Shatrah, Thi-Qar, Iraq; 2Department of Physiology, College of Veterinary Medicine and Surgery, Shatrah University, Shatrah, Thi-Qar, Iraq.

*Correspondence | Abbas Kamil Shlaga, Department of Microbiology, College of Veterinary medicine and Surgery, Shatrah University, Shatrah, Thi-Qar, Iraq; Email: [email protected]

Figure 1

Clinical signs and postmortem symptoms of infected birds with NDV (A) paralysis of legs and wings, twisting of neck due to nervous system infection; (B) respiratory signs and paralysis; (C) proventriculus haemorrhage (D) cecal haemorrhage (D) haemorrhage and congestion of intestine (F) enlargement and congestion of liver and spleen. 

Figure 2

Antibody titer infected birds and control group. 

Figure 3

Amplification plot for matrix gene of Newcastle disease virus by RT-PCR in naturally infected chicks. 

Figure 4

Changes in the mRNA levels for the inflammatory biomarkers in the infected birds comparing with non-infected birds: A=CRP, B=IFN-γ, C=IL6 and D=IL-1β. 

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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