Effect of hcp1 of the Type VI Secretion System on Biological Characteristics of F18 Shiga Toxin-Producing Escherichia coli
Effect of hcp1 of the Type VI Secretion System on Biological Characteristics of F18 Shiga Toxin-Producing Escherichia coli
Yang Yang1,2, Hong Zhou3, Ji Shao1,2, Xinyi Zhang1,2, Pengpeng Xia1,2, Mingxu Zhou4,5, Qiangde Duan1,2 and Guoqiang Zhu1,2*
Fig. 1.
Results of growth curve (A) and motility assay (B).
Notes: A, there was no significant difference in growth rate among the three strains. B, there was no significant difference in biofilm formation ability among the three strains.
Fig. 2.
The motility, adherence and colonization assays of bacterial. A, Flagella related motility assays. A1: F18ab; A2: F18abΔhcp1; A3: F18abΔhcp1/phcp1. B, Adherence ability towards IPEC-J2 cell line. C, Invasion ability towards IPEC-J2 cell line. D, Intestinal colonization test. * Significant difference, p<0.05.
Fig. 3.
Vero cell cytotoxicity assay.
* Significant difference, p<0.05.
Fig. 4.
Transcriptional levels of virulence related genes.
*: The gene transcriptional levels compared with F18ab, P<0.05
October 2022
Vol. 54, Iss. 5, Pages 2003-2500
