Effect of Prokaryotic Expressed Nucleoplasmin on the Efficiency of Somatic Cell Nuclear Transfer in Banna Mini-pig Inbred Line
Effect of Prokaryotic Expressed Nucleoplasmin on the Efficiency of Somatic Cell Nuclear Transfer in Banna Mini-pig Inbred Line
Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
The PCR results of the Npm. M, marker.
SDS-PAGE analysis of the pCold II-INPM2 protein expression. A, transformed E. coli BL21 (DE3) cells and induced with 0.5 mM IPTG; B, transformed E. coli BL21 (DE3) cells and induced with 0.1mM IPTG. Lane A, non-induced crude; Lane B, Induced crude; LaneB1-B4, Induced crude; Lane C, Supernatant of lysate; Lane D, Precipitation of lysate. MK, Molecular weight marker.
SDS-PAGE analysis of the pCold II-INPM2 protein expression. A, transformed pG-TF2 expression strains and induced with 0.5mM IPTG; B, transformed pG-TF2 expression strains and induced with 0.5mM IPTG. Lane A, non-induced crude; Lane B, Induced crude; LaneB1-B4, Induced crude; Lane C, Supernatant of lysate; Lane D, Precipitation of lysate. MK, Molecular weight marker.
Protein was concentrated in D and E lanes by chelating SFF (Ni) column separation and purification.
Protein product. Buffer (20 mM PB, 500 mM NaCl, pH 7.4).
