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Diversity of Extended Spectrum β-lactamases among Multi Drug Resistant Clinical Isolates of Pseudomonas aeruginosa Collected fromTertiary Care Hospitals of Peshawar, Pakistan

Diversity of Extended Spectrum β-lactamases among Multi Drug Resistant Clinical Isolates of Pseudomonas aeruginosa Collected fromTertiary Care Hospitals of Peshawar, Pakistan

Amjad Ali, Kafeel Ahmad*and Shaista Rahat

Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan

*      Corresponding author: kafeelpbg@gmail.com

Fig. 1.

Double Disc Synergy Test: ESBL positive Pseudomonas aeruginosa, the size of zones of inhibition around cefepime, ceftazidime and aztreonam are enhanced towards center amoxicillin-clavulanic acid disc as shown by arrows.

Fig. 2.

A, PCR amplification of blaOXA-10 gene (A) Lane 1 = 100 bp DNA ladder. Lanes 4, 5, 8, 12, 13, 14 and 16 in A show blaOXA-10 (760 bp) blaCTX-M gene; (B), Lanes 3, 8, 11, 12, 13, 15 and 16 in B show blaCTX-M (552 bp) positive blaTEM gene (C), Lanes 3 and 16 show blaTEM (856 bp) positive blaSHV gene and (D) Lanes 2, 3 and 4 in D show blaSHV (230 bp) positive Pseudomonas aeruginosa isolates.

Fig. 2.

A, PCR amplification of blaOXA-10 gene (A) Lane 1 = 100 bp DNA ladder. Lanes 4, 5, 8, 12, 13, 14 and 16 in A show blaOXA-10 (760 bp) blaCTX-M gene; (B), Lanes 3, 8, 11, 12, 13, 15 and 16 in B show blaCTX-M (552 bp) positive blaTEM gene (C), Lanes 3 and 16 show blaTEM (856 bp) positive blaSHV gene and (D) Lanes 2, 3 and 4 in D show blaSHV (230 bp) positive Pseudomonas aeruginosa isolates.

Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

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