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Differential Cytokine Expression Analyses of Cefs and Defs Infected with H6 Influenza Virus Strains Isolated from Anseriformes in Anhui Province, China

Differential Cytokine Expression Analyses of Cefs and Defs Infected with H6 Influenza Virus Strains Isolated from Anseriformes in Anhui Province, China

Ye Ge1,2,3, Qiucheng Yao1, Hongliang Chai3, Yuping Hua3* and Guohua Deng2*

1College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong Province, China
2State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
3College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang Province, China

*      Corresponding author: dgh1971@163.com; yuping_hua@126.com

Fig. 1.

Multi-cycle replication of H6 avian influenza viruses in A549 cells. A549 monolayers were inoculated with virus at a MOI of 0.01, and the culture supernatants were collected at the indicated time points and then titrated in eggs.

Fig. 2.

Expression of pro-inflammatory factors (IL-6 and IL-8), antiviral cytokines (IFN-α and IFN-β), an anti-inflammatory cytokine (IL-10) and a pathogen recognition receptor (TLR7) in CEFs and DEFs. Monolayer cells were collected at 0, 2, 4, 8, 12, 24, 36 h after infection. For each cytokine, the expression data were normalized to GAPDH expression. The column data were calculated by -△△Ct. The results are presented as the mean fold change±SD. Error bars represent standard error.

Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

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