Differential Cytokine Expression Analyses of Cefs and Defs Infected with H6 Influenza Virus Strains Isolated from Anseriformes in Anhui Province, China
Differential Cytokine Expression Analyses of Cefs and Defs Infected with H6 Influenza Virus Strains Isolated from Anseriformes in Anhui Province, China
Ye Ge1,2,3, Qiucheng Yao1, Hongliang Chai3, Yuping Hua3* and Guohua Deng2*
Fig. 1.
Multi-cycle replication of H6 avian influenza viruses in A549 cells. A549 monolayers were inoculated with virus at a MOI of 0.01, and the culture supernatants were collected at the indicated time points and then titrated in eggs.
Fig. 2.
Expression of pro-inflammatory factors (IL-6 and IL-8), antiviral cytokines (IFN-α and IFN-β), an anti-inflammatory cytokine (IL-10) and a pathogen recognition receptor (TLR7) in CEFs and DEFs. Monolayer cells were collected at 0, 2, 4, 8, 12, 24, 36 h after infection. For each cytokine, the expression data were normalized to GAPDH expression. The column data were calculated by -△△Ct. The results are presented as the mean fold change±SD. Error bars represent standard error.
April 2021
Vol. 53, Iss. 2, Pages 401-800
