Diafenthiuron Adversely Affects the Complete Blood Count, Serum Biochemical Profile and Antioxidants in Vital Organs of Ctenopharyngodon idella
Diafenthiuron Adversely Affects the Complete Blood Count, Serum Biochemical Profile and Antioxidants in Vital Organs of Ctenopharyngodon idella
Asher Azeem1, Misbah Javed2, Muhammad Riaz ul Haq1, Maria Shahzeen1, Muhammad Asif1, Gul Muhammad1, Khalid Hussain3, Ahmad Ali3, Muhammad Latif4, Noreen Samad2 and Furhan Iqbal1*
ABSTRACT
Diafenthiuron is the most common used pesticide in Pakistan that causes insect pest mortality by mitochondrial function inhibition. It has been reported in local news papers that this pesticide is entering in our fresh water bodies affecting aquatic life. The objective of this investigation was to report the LC50 value of diafenthiuron for grass carp, Ctenopharyngodon idella and to report the effect of various doses such as 0.0038 (T1), 0.05 (T2), 0.5 (T3), 1 (T4), 5 (T5) and 6.67 mg/L (T6) on complete blood count, serum biochemical profile and antioxidant parameters in kidney and liver of this fish following 96 h exposure. An untreated control group was maintained in parallel. Our results indicated that 96 h LC50 value of diafenthiuron for juvenile C. idella was 5.67 mg/L. Analysis of the hematological profile revealed that red blood cell (RBC) count, haematocrit, mean corpuscular hemoglobin concentration, hemoglobin, white blood cells, monocytes, granulocytes and lymphocytes significantly increased while RBC distribution width, mean corpuscular haemoglobin, mean corpuscular volume and platelet distribution width significantly decreased in diafenthiuron exposed treatments than control group. Significant increase in serum triglycerides level was observed in C. idella treatments exposed to diafenthiuron. Concentrations of catalase, superoxide dismutase and malondialdehyde also varied significantly in liver and kidney when compared between diafenthiuron treated and untreated C. idella. In conclusion, we are reporting that diafenthiuron can adversely affect the hematological, serum and antioxidant parameters of non-target organism like C. idella and its entry in water bodies must be prevented in order to conserve our fresh water ecosystems and food web.
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