Detection and Phylogenetic Analysis of Infectious Bursal Disease Virus Based on both Genome Segments A and B Isolated from Backyard Poultry Punjab, Pakistan
Detection and Phylogenetic Analysis of Infectious Bursal Disease Virus Based on both Genome Segments A and B Isolated from Backyard Poultry Punjab, Pakistan
Syeda Fakhra Waheed1, Asim Aslam1*, Muti-ur-Rehman Khan1, Kamran Ashraf2 and Beenish Zahid3
Evolutionary tree constructed from segment B-coded VP1 gene sequences reported in different geographical regions of the world with present study isolates highlighted with blue color
Evolutionary tree constructed from segment A-coded VP2 gene sequences reported in different geographical regions of the world with present study isolates highlighted with blue color.
Mean pairwise amino acid identity of present IBDV isolate with previous strains VP1 gene based sequence alignment revealed presence of characteristic amino acids of non vvIBDV at position 146-E, 147-G, 242-D, 390-L, 508-k, 511-S. characteristic amino acids of very virulent IBDV at position 287-A.
Mean pairwise amino acid identity of present IBDV isolate with already reported strains. VP2 gene based sequence alignment revealed presence of characteristic amino acids of vvIBDV at position 222-A, 242-I, 253-Q, 256-I, 279-D, 284-A, 299-S, 330-S and (384-I a distinct AA).
