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Bovine Tuberculosis (bTB): Detection of Mycobacterium bovis Infection with Specie-specific Primers in Sputum Samples in Pakistan

Bovine Tuberculosis (bTB): Detection of Mycobacterium bovis Infection with Specie-specific Primers in Sputum Samples in Pakistan

Asad Ullah1*, Umar Sadique2, Ibadullah Jan2, Imad Khan1, Raheela Taj3, Mumtaz Ali Khan4, Salah-ud-din4, Naimat Ullah Khan1

1College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Pakistan; 2College of Veterinary Sciences, The University of Agriculture Peshawar, Khyber Pakhtunkhwa, Pakistan; 3Institute of Chemical Sciences (ICS), The University of Peshawar, Pakistan; 4University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan.

 
*Correspondence | Asad Ullah, College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Pakistan; Email: [email protected]

Figure 1:

Sample collection site, district Peshawar Khyber Pakhtunkhwa (KP) province. Pakistan.

Figure 2:

Graph showing the overall results of Ziehl-Neelsen staining, Culture and PCR of the occupational groups in the study area.

Figure 3:

PCR amplicon showing specie-specific 500bp DNA of Mycobacterium bovis (S5, 7-12 and S14). S1-4, 6 & 13 are negative samples.

Figure 4:

Ziehl-Neelsen staining of sputum samples of TB patients showing acid-fast bacilli.

Figure 5:

Ziehl-Neelsen staining of sputum samples of livestock farm workers showing acid-fast bacilli.

Figure 6:

Ziehl-Neelsen staining of sputum samples of abattoir workers showing acid-fast bacilli.

Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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