Bovine Tuberculosis (bTB): Detection of Mycobacterium bovis Infection with Specie-specific Primers in Sputum Samples in Pakistan
Asad Ullah1*, Umar Sadique2, Ibadullah Jan2, Imad Khan1, Raheela Taj3, Mumtaz Ali Khan4, Salah-ud-din4, Naimat Ullah Khan1
1College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Pakistan; 2College of Veterinary Sciences, The University of Agriculture Peshawar, Khyber Pakhtunkhwa, Pakistan; 3Institute of Chemical Sciences (ICS), The University of Peshawar, Pakistan; 4University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan.
*Correspondence | Asad Ullah, College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Pakistan; Email:
[email protected]
Figure 1:
Sample collection site, district Peshawar Khyber Pakhtunkhwa (KP) province. Pakistan.
Figure 2:
Graph showing the overall results of Ziehl-Neelsen staining, Culture and PCR of the occupational groups in the study area.
Figure 3:
PCR amplicon showing specie-specific 500bp DNA of Mycobacterium bovis (S5, 7-12 and S14). S1-4, 6 & 13 are negative samples.
Figure 4:
Ziehl-Neelsen staining of sputum samples of TB patients showing acid-fast bacilli.
Figure 5:
Ziehl-Neelsen staining of sputum samples of livestock farm workers showing acid-fast bacilli.
Figure 6:
Ziehl-Neelsen staining of sputum samples of abattoir workers showing acid-fast bacilli.