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Bovine Tuberculosis (bTB)-Isolation and Species-Specific Identification of Mycobacterium bovis from Bovine Raw Milk in Pakistan

Bovine Tuberculosis (bTB)-Isolation and Species-Specific Identification of Mycobacterium bovis from Bovine Raw Milk in Pakistan

Asad Ullah1*, Umar Sadique2, Sultan Ayaz1, Muhammad Subhan Qureshi2 and Farhan Anwar Khan

1College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Pakistan; 2College of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, KPK, Pakistan.

asadullah@awkum.edu.pk  

Figure 1

PCR result of bovine milk samples showing specie specific amplicon size of 500bp DNA of Mycobacterium bovis (M.bovis). S1-4 are M. bovisnegative samples and S5-6 are M. bovispositive samples. 

Figure 2

Ziehl-Neelsen staining showing acid-fast bacilli from milk samples 

Figure 3

Ziehl-Neelsen staining showing acid-fast bacilli from milk samples 

Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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