Bacillus subtilis BML5 Isolated from Soil Contaminated with Poultry Waste has Keratinolytic Activity
Bacillus subtilis BML5 Isolated from Soil Contaminated with Poultry Waste has Keratinolytic Activity
Aqsa Imtiaz and Abdul Rehman*
Fig. 1.
Growth curves of B. subtilis BML5 in medium containing glucose (control) and in mineral salt medium supplemented with 1% feathers as substrate (treated) incubated at 37°C.
Fig. 2.
Effect of temperature (A), pH (B) and metal ions (C) on keratinase activity isolated from B. subtilis BML5.
Fig. 3.
Coomassie Brilliant blue-stained SDS-PAGE of partially purified keratinase from B. subtilis BML5. Lane C, bacterium growing without feather; Lane T, bacterium growing with feather; Lane M, molecular mass protein maker.
Fig. 4.
Degradation of feathers by B. subtilis BML5 culture in minimal salt medium after 6 days of incubation at its optimum temperature.
February 2018
Vol. 50, Iss. 1, Pages 1-400
