Assessment if Time-Alteration in the Fixative Procedure of Five Types of Fixatives on the Output Precision and Digital Analysis of Tissue Architecture Images
Assessment if Time-Alteration in the Fixative Procedure of Five Types of Fixatives on the Output Precision and Digital Analysis of Tissue Architecture Images
Alaa H. Sadoon1, Zainab Abdulimam Majeed2, Majdy Faisal Majeed1*
shows the percentage % of hard and cut (a) and crumbling sections of samples according to an alteration in fixation protocol (different fixations and time).
Showed histological liver specimens were fixed in NBF (10%) for 6 hr. (A), 12 hr. (B), and 24 hr. (C). Fixed in Bouin’s for 6 hours (D), 12 hours (E), and 24 hours (F). Fixed in Carony,s for 6 hours (G) and 12 hours (H). In Zenker,s 12 hours (I) and 24 hours (J). In Clarke’s 6 hours (K) and 12 hours (L), staining of H and E 400X.
Photomicrographs (400X) of liver tissue specimens were image-analyzed using Image J software as a percentage of quantification for nuclei and cell cytoplasm (a, b, c, d, e, and f). The samples were fixed in formaldehyde for 12, 24, and 48 hours. While (g, h, I, j, k, and l) were fixed in Bouin’s fluid for 12, 24, and 48 hours, and (m, n, p, and q) were fixed in Carony,s fluid for 12 and 24 hours.
The overall histological score shows the staining score and histological deformity (shrinkage, swelling, and distortion/dissolution) of the nuclear and cytoplasmic as a comparison of various fixatives and intervals of time with alteration of procedure for standard fixation criteria.
