Assessment on Coffee Cherry Flour of Mengani Arabica Coffee, Bali, Indonesia as Iron Non-Heme Source

Roy Hendroko Setyobudi1, Erkata Yandri2, Yogo Adi Nugroho3, Mardiana Sri Susanti4,

Satriyo Krido Wahono5, Wahyu Widodo1, Lili Zalizar1, Elfi Anis Saati1, Maftuchah Maftuchah1,

Manar Fayiz Mousa Atoum6, Muhannad Illayan Massadeh6, Dwi Yono3, Rangga Kala Mahaswa7,

Herry Susanto2, Damat Damat1*, Dyah Roeswitawati1, Praptiningsih Gamawati Adinurani8 and

Susi Mindarti1

1University of Muhammadiyah Malang, Indonesia; 2Darma Persada University, Jakarta, Indonesia; 3IPB University, Bogor, Indonesia; 4Aura Statistics Consultant, Malang, Indonesia; 5Research Division for Natural Product Technology (BPTBA), National Research and Innovation Agency Republic of Indonesia (BRIN), Yogyakarta, Indonesia; 6The Hashemite University, Jordan; 7Universitas Gadjah Mada, Yogyakarta, Indonesia; 8Merdeka University of Madiun, Indonesia.

Received | November 01, 2021; Accepted | December 28, 2021; Published | December 30, 2021

*Correspondence | Damat Damat, Department of Food Technology, Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang, Jl.Raya Tlogomas No.246, Malang, 65144, Indonesia; Email: [email protected]

Citation | Setyobudi, R.H., E. Yandri, Y.A. Nugroho, M.S. Susanti, S.K. Wahono, W. Widodo, L. Zalizar, E.A. Saati, M. Maftuchah, M.F.M. Atoum, M.I. Massadeh, D. Yono, R.K. Mahaswa, H. Susanto, D. Damat, D. Roeswitawati, P.G. Adinurani and S. Mindarti. 2021. Assessment on Coffee Cherry Flour of Mengani Arabica Coffee, Bali, Indonesia as Iron Non-Heme Source. Sarhad Journal of Agriculture, 37(Special issue 1): 171-183.

DOI | https://dx.doi.org/10.17582/journal.sja/2022.37.s1.171.183

Keywords | Anemia herbal medicine, Circular economy, Environmentally-friendly, Fe non-heme, Waste to functional food

Introduction

The International Coffee Organization (ICO) – held in July 2019 – reported that the global coffee production in 2018 had grown up to 10.29 % compared to 2015. However, another note from the same organization was that the world’s coffee consumption increased 6.52 % within the same time span (Shahbandeh, 2020). Considering how the consumption rate is lower than the production one, it is obvious that the world has quite a surplus, causing pressure toward coffee prices (Murphy, 2019). Furthermore, Acosta (2019) revealed how Columbia had been losing up to 40 000 ha of coffee plantation within 18 mo (months) in 2018 after its farmers opted to discontinue tending due to sliding prices of coffee.

Further, the COVID-19 pandemic strike impacts a large number of closed cafés due to lockdown and semi-lockdown policies, lowering the sales number of green beans coffee (Al-Fadly, 2020; Kurniawan, 2020). Furthermore, Kevon et al. (2021) predicted the decrease of coffee production globally in 2022 – which would go further south as long as the pandemic was still around – since coffee plants could not be properly tended. 

Coffee stakeholders need to overcome the above problem and efficiency should be a choice. James and Deswarte (2015) and Stéphane and Thomas (2009) advised applying biorefinery, i.e. to process waste and turn it into economical products. Several studies on coffee waste utilization have been done by Padmapriya et al. (2019), Pushpa and Naidu (2012), Pushpa et al. (2012), and Setyobudi et al. (2018). Setyobudi et al. (2019) specifically proposed recycling coffee pulps (CP) and coffee husks (CH) into coffee cherry flour (CCF). This process gives double benefits since it is not only to minimize CP and CH pollutants (Elida, 2012; Grisel et al., 2014; Melkayo et al., 2016) but also to generate highly economical products (Ebba, 2015; Hermosa et al., 2014; Sukrisno, 2013).

Andrew Fedak and Dan Belliveau introduced the pulp and husk-based Coffee Flour at TED Conference 2015, Vancouver, Kanada (Deborah, 2021; Emily, 2015), later they patented it as Coffee Cherry Flour (CCF) (Rachel, 2019; WIPO–PCT, 2014). CCF is registered in Trademarkia dated August 9, 2015, under Serial Number of 86245457 and Registration Number of 4806487. Global Holdings, Inc. (2015) stated that CCF contains 84 % lower fat and 42 %

higher fibre than coconut flour, 38 % higher antioxidant than pomegranate (Punica granatum L.), more than three times than iron (Fe) in fresh spinach (Spinacia oleracea L.), five times higher than fibre of wheat flour, more than three times than protein in kale (Brassica oleracea var. sabellica L.), and more than twice than potassium of banana (Musa paradisiaca L.).

In 2014/2015, CCF has been made in Nicaragua, Guatemala, Mexico, and Vietnam, and more will be produced in Brazil, Columbia, and El Salvador (Global Holding, Inc., 2015). As the fourth biggest coffee producer globally, Indonesia (Rahardjo et al., 2020; Van Der Schaar Investments, 2019) was overlooked by Global Holding regarding the CCF production base plan. 

Damat et al. (2019), Elba et al. (2017), Mindarti et al. (2020), Moreno et al. (2019) and Rosas-Sánchez (2021), has discussed CCF’s positive impacts on the fibre content; however, the study on CCF as Fe source has not yet been discovered. Coffee Cherry Co. (2021) reported that about 49.6 mg 100 g–1 (Figure 1a), while, Global Holdings, Inc. (2015) found that the content of Fe in CCF was about 13 % (Figure 1b). The Fe content is higher than one in S. oleracea, ranging from 2.7 mg 100 g–1 to 3.9 mg 100 g–1 (DKBM Indonesia, 2017; USDA, 2018).

The possibility of finding Fe sources from CP and CH as inexpensive haemoglobin booster is beneficial to research (Marín-Tello, 2020) due to the relatively high chance of anemia (Milman, 2011; Nurbadriyah, 2019), particularly in Indonesia (Bukhari et al., 2020; Ellie et al., 2012). Das and Das (2020) and Petek et al. (2020) highlighted how anemia impacted COVID acuteness. Several research on haemoglobin boosters have been studied, i.e. S. oleracea (Dheny and Umarianti, 2017; Nasution et al., 2021), Moringa olifera Lam. (Indriani et al., 2019; Tri and Sunarsih, 2021), and Phoenix dactylifera L. (Nur and Maulany, 2021; Resti et al., 2021). This study aims to determine whether CCF made in Indonesia can meet the quality standard of commercially available products as Fe source.

Materials and Methods

Coffee pulp

Coffee Pulp (CP) as fresh solid waste was obtained from Pabrik Pengolahan Kopi (PK) Arabika, Mengani, a coffee processing company in Kintamani, Bangli, Bali (coordinate 8° 17’ 16.63” S 115° 15’ 0.61” E). Kintamani, Bali was appointed as research site due to two reasons: (i) Arabika Kintamani Bali is first Indonesia’s Geographical Indication goods registered in 2005 and enlisted on December 5, 2008, therefore numbered ID G 000000001 (Nizar, 2018); and (ii) PK Arabika, Mengani is one of Indonesian coffee processing companies awarded the Gold Gourmet Agency for the Valorization of Agricultural Products (AVPA) in the World Roast Coffee Competition 2018 in Paris (Ferry, 2018).

 

CP (Coffea arabica L.) samples were randomly picked from the pulper during May to June 2018 production. CP with 70 % to 85 % water contents were drained under the shade and oven-dried (Maksindo cabinet dryer) at 80 °C for 15 h, and only 5 % water content remained. CP was then ground into CCF using Eureka Mignon Coffee Grinder at the speed of 1 350 rpm (1 rpm = 1/60 Hz) and stored in plastic bags. 

The CCF commercial product (as a comparator) from La Boite, a store at Manhattan, 724 11th Avenue New York, NY 10019, was bought in May 2018. The CCF had been made in Brazil.

Fe Analysis

The Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) Varian-730-ES by a Varian Inc under the Agilent Technologies (Palo Alto, USA) was employed to perform the Fe mineral content analysis. A total of 5 g CCF of samples were used by the dry-ashing procedures with hydrochloric acid dilution to extract the minerals, then analyzed in duplicates from each sample and replicated at least twice times (Xiandeng et al., 2016). 

Vitamin C Analysis

The Iodimetric method (AOAC, 1995) was used in Vitamin C (Vit. C) analysis. A total of 5 g CCF of samples were diluted in a 100 mL flask and marked. The dilutions were then filtered, and 25 mL of each was then mixed with a few drops of starch and quickly titrated with 0.01N sodium thiosulfate to blue color. Vit. C content was calculated based on Equation (1):

Vit. C (mg/100g) = (V I2 × 0.88 × Fp) × 100 W s (1)

Where;

V I2 = Iodium Volume (mL), NFp = Dilution Factor, W s = Sample weight (g).

Amino acid, total carotene, and glucose and fructose

The amino acid composition of the major ingredients was determined by an automated amino acid analyzer (Hi-Tachi, Tokyo, Japan) following acid hydrolysis with 6 N HCl (reflux for 23 h at 110 °C), according to Qiu et al. (2016) and Zmrhal et al. (1975). Amount 0.1 g of samples were solved and homogenized with acetone, followed by a UV-Vis spectrophotometer reader at λ = 450 nm to determine Carotene content, which was estimated according to the absorbance of samples at the standardized linear curve (Machmudah and Goto, 2013; Rodriguez-Amaya and Kimura, 2004). Glucose and Fructose contents were determined by the colorimetry method (DuBois et al., 1956).

Statistical analysis

Utilizing descriptive statistics, the result is presented in box-plot in accordance with the Student’s t-test 

α = 0.05. Statistical significance for differences between samples was determined by the GLM-ANOVA (Ramesh et al., 2019).

Results and Discussion

Fe content

Results on Fe contents of both Mengani’s CCF and La Boite’s are presented in Figure 2.

Figure 2 shows that Fe in median (Q2) La Boite’s CCF is 183.730 mg kg–1, which is higher than in median (Q2) Mengani’s CCF of 92.687 mg kg–1 but statistically (P > 0.0.5) insignificant. Although relatively low, Fe in Mengani’s CCF (9.269 mg 100 g–1) includes a range of CP or CH [(4.3 to 15) mg 100 g–1], referring to Iriondo-DeHond et al. (2020).

The low Fe content in Mengani’s CCF corroborates data compiled by Setyobudi et al. (2018). Kintamani land is categorized as Inceptisol (Asfimanto et al., 2013; Nurul et al., 2022), young land after the Mount Batur volcanic eruption. Some references mentioned by Hasibuan et al. (2014) and Nandini and Narendra (2012) stated that Inceptisol characteristics as pH being acidic to slightly acidic and soil electrical conductivity (EC) being shallow, whereas organic matter, total nitrogen, cation exchange capacity (CEC), saturation bases, potassium potency, and iron hydrous oxide low. Nandini and Narendra (2012) also found that the Fe content of Bangli, Kintamani soil was in the range of 15.0 mg kg–1 to 18.4 mg kg–1, which was categorized as very low.  

 

Box plot in Figure 2 shows Fe content in Mengani’s CCF with Q1 = 47.166 mg kg–1, Q2 = 92.687 mg kg–1, and Q3 = 156.86 mg kg–1. Interquartile Range (IQR) depicts how Fe data is dispersed and asymmetrical since Q2 tends to go downwards (Q1) or slant rightwards. However, the distribution is considered normal since the tilt is not too drastic (0.299 %), and there is no apparent Outlier. As for Fe content in La Boite’s CCF – Q1 = 109.14 mg kg–1, Q2 = 183.73 mg kg–1,

and Q3 = 258.87 mg kg–1 – IQR comes out with dispersed but symmetrical data. 

Comparing Figure 1 and Figure 2, the Fe content in the latter is lower. It is then presumed that the claims made by CCF producers (Global Holdings, Inc., 2015) of 13 % = 23.4 mg 100 g–1 (FDA, 2020) and 49.6 mg 100 g–1 (Coffee Cherry Co., 2021) were “overestimated” (especially Figure 1a). Other studies also found variously “lower Fe contents” in CP or CH. Kayhanian et al. (2016) found ones between the ranges of (10 to 50) mg 100 g–1. Wich (2015) reported it as 10 mg 100 g–1. Iriondo-DeHond et al. (2020) came up with (4.3 to 15) mg 100 g–1 in their review article referring to five studies. Marín-Tello et al. (2020) concluded that to be 7.70 mg 100 g–1.

Elias (1979) went with 15 mg 100 g–1. Anna et al. (2019) discovered 15 mg 100 g–1. Zupancic and Grilc (2012) declared 25 mg 100 g–1. Avinash et al. (2017) came across about 28.7 mg 100 g–1 in the fresh CP. Setyobudi et al. (2018) found that CP from Kintamani-Bali, Indonesia, processed as hay with

15 mo shelf life, contained Fe of 13.9 mg 100 g–1.

Fe in Mengani’s CCF is indeed lower than La Boite’s; yet, the content of 9.269 mg 100 g–1, ranged (4.717 to 15.686) mg 100 g–1 is still higher than in spinach (S. oleracea.). DKBM Indonesia (2017) stated that Fe content in S. oleracea was 3.9 mg 100 g–1, while the USDA (2018) mentioned that about 2.7 mg 100 g–1.

It is higher than several other vegetable sources, such as Sauropus androgynus L. (Merr), Manihot esculenta Crantz leaf, Carica papaya L. leaf, Brassica juncea L., Solanum lycopersicum L., and Daucus carota subsp. Sativus (Hoffm.) Schubl. & G. Martens. (Setyobudi et al., 2019). It is even higher than the current study of haemoglobin booster, i.e. M. oleifera 

(7 mg 100 g–1) and Phoenix dactylifera L. [(4.06 to 7.06) mg 100 g–1] (DKBM Indonesia, 2017; Rania et al., 2014).

Fe nutrition is divided into heme (Ferro, Fe2+ bond, generally animal-based food) and non-heme (Ferri Fe3+ bond, generally plant-based food). Human body absorption of non-heme Fe is relatively low, around 5 % only, while heme Fe can be absorbed between 10 % to 30 %. Therefore, nutrition elements in Fe should be taken in more easily if enhancer (booster or promoter) agents are available (Ari, 2014, Jumadi, 2020, Nurbadriyah, 2019; Setyobudi et al., 2019). 

Vitamin C

Vitamin C (Vit. C), with its capability to reduce Ferri (Fe3+) into Ferro (Fe2+) in the small intestine, helps to increase non-heme Fe absorption. It is also keen to prevent haemosiderin from forming, which is beneficial since it is difficult to mobilize in iron release when needed (Pratiwi, 2015). Several research teams have proven the role of Vit. C as an enhancer agent, e.g., Conner et al. (2012) and Agusmayanti et al. (2020).

Vit. C contents in Mengani’s CCF and La Boite’s are detailed in Figure 3.

 

Figure 1, Coffee Flour BJ (2021) and Wich (2015) have put 0 (zero) content of Vit. C in CCF. In spite of that, this research has recorded median Vit. C in Mengani’s CCF to be 185.45 mg 100 g–1 and median Vit. C in La Boite’s CCF to be 194.6 mg 100 g–1 in Figure 3, which is statistically insignificant (P > 0.05) when compared. Box-plot of Mengani’s CCF shows Q1 =143.03 mg 100 g–1, Q2 = 185.45 mg 100 g–1, dan Q3 = 227.27 mg 100 g–1 with evenly-spread data. Similarly, IQR of La Boite’s CCF comes out with evenly-spread data with Q1 = 153.33 mg 100 g–1, Q2 = 194.6 mg 100 g–1, and Q3 = 234.55 mg 100 g–1.

Figure 3 is in sync with Sukartiningsih (2011) ‘s conclusion that the cascara (pericarp, exocarp, and mesocarp) of fresh before-ripe Arabica coffee contains Vit. C = (275.7 to 651.2) mg 100 g–1. 

Despite insignificance in statistics, Mengani’s CCF tends to have lower Vit. C. Different CP or CH drying method is inferred to be the cause. While Mengani’s was non-naturally dried at a constant temperature of 80 °C for 15 h, La Boite’s was naturally dried under the sun on the drying floor (CGTN America, 2014). Mahendra et al. (2014) and Usamah et al. (2019) informed that tropical drying floors are typically 22.67 °C to 37.90 °C with inconsistent temperatures – therefore, goods must be turned over every 1 h to

2 h – and drying time of approximately 7 h d–1.

Vit. C in Mengani’s CCF = 185.45 mg 100 g–1 [ranged (143.03 to 227.27) mg 100 g–1], which is higher than Iriondo-DeHond et al. (2020) ‘s data of 69.80 mg 100 g–1. Furthermore, it is superior compared to other haemoglobin booster plants, such as S. oleracea 

(80 mg 100 g–1), M. oleifera (fresh = 220 mg 100 g–1, dried = 17.3 mg 100 g–1), and P dactylifera [(0.7 to 0.9) mg 100 g–1] (DKBM Indonesia, 2017; Egbuna, 2015; Sultana et al., 2015).

Comparing the rates of Vit. C in fresh M. oleifera to in dried one (Egbuna, 2015) as well as in fresh coffee cascara (Sukartiningsih, 2011) as shown in Fig. 3, it is safe to deduce that a low-temperature drying process is essential to preserve the substance in CP. Alakali et al. (2015), Kang et al. (2007), and Rizki et al. (2018) also supported the using low temperature.

Vitamin A

Vitamin A (Vit. A) is also able to increase non-heme Fe absorption, as research done by Damayanti et al. (2016) and Fernanda et al. (2013). Figure 4 points out Vit. A (carotenoid) content in Mengani’s CCF and La Boite’s.

Figure 4 illustrates that carotenoid in median (Q2) Mengani’s CCF is of 4.370 g mg–1, which is lower than in median (Q2) La Boite’s of 4.435 g mg–1 but statistically (P > 0.05) insignificant. The box-plot shows carotenoid in Mengani’s CCF of Q1 = 4.370 g mg–1, Q2 = 4.370 g mg–1, dan Q3 = 4.435g mg–1 with centralized data. La Boite’s IQR shows Q1 = 4.367 g mg–1, Q2 = 4.435g mg–1, dan Q3 = 4.587 g mg–1 with evenly-spread data.

Resembling the Vit. C result, Mengani’s CCF tends to have lower carotenoids due to the CP or CH drying process, although it statistically insignificant. Alakali et al. (2015), Ayegba et al. (2017), Kurniawati (2016) and Meiliana et al. (2014) have demonstrated in their studies how high temperature degrades carotenoid.

The carotenoid median in Mengani’s CCF, as recorded in Figure 4, is 4.370 g mg–1, ranging (4.367 to 4.587) g mg–1 – it is higher than the same nutrition contained in S. oleracea, M. oleifera, and P dactylifera (Alakali et al., 2015; Kemenkes, 2018; Priyo et al., 2020; Sultana et al., 2015).

 

Amino acid

Ari (2014) and El-Hawary et al. (1975) have confirmed the role of protein as an enhancer agent in the Fe absorption process. Comparison of total amino acid contents in Mengani’s CCF and La Boite’s are presented in Figure 5.

 

Figure 5 shows the median (Q2) of total amino acid in Mengani’s CCF to be 7.571 g 100 g–1, which is statistically (P > 0.01) of significant difference compared to the median (Q2) of total amino acid in La Boite’s CCF = 10.735 g 100 g–1. The box plot lists total amino acid content in Mengani’s CCF to be Q1 = 7.222 g 100 g–1, Q2 = 7.571 g 100 g–1, and Q3 =7.92 g 100 g–1, with symmetrical, evenly-spread data. Meanwhile, the IQR of La Boite’s CCF is Q1 = 10.625 g 100 g–1, Q2 =10.735 g 100 g–1, and Q3 = 10.822 g 100 g–1 with centralized dispersion. Overheating affects amino acids, as it considerably degrades the one in Mengani’s CCF. Oestreich (2013) stated that the amino acid content in roasted arabica and robusta beans is lower than green beans. Ika et al. (2015) also reported decreases in amino acids, fatty acids, cholesterol, and mineral composition in eel (Monopterus albus Zuiew, 1793) due to deep frying. Ayegba et al. (2017) came up with further information on the dwindling percentage of protein in M. oleifera related to high temperature.

Fructose and Glucose

Ari (2013) and Christides and Sharp (2013), declared that sugar is also an enhancer agent for non-heme Fe. Researchers Nur and Maulany (2021) and Resti et al. (2021) proved that even with relatively little Fe in P. dactylifera – amounted (4.06 to 7.06) mg 100 g–1

(Rania et al., 2014) – the fruit was able to boost haemoglobin because of sugar as an enhancer. Nadeem et al. (2019) catalogued that P. dactylifera

contained glucose 26.75 % to 44.44 %, fructose

21.03 % to 33.21 %, and sucrose 4.17 % to 6.32 %. Fructose and glucose contents of both Mengani’s CCF and La Boite’s are disclosed in Figure 6.

 

Figure 6 shows the median (Q2) of fructose in Mengani’s CCF to be 1 997.80 mg kg–1, which is statistically (P > 0.01) of significant difference compared to the median (Q2) of fructose in La Boite’s CCF = 1 533.33 mg kg–1. The box plot lists total fructose content in Mengani’s CCF to be Q1 = 1 877.80 mg kg–1, Q2 = 1 997.80 mg kg–1, and Q3 = 2 088.90 mg kg–1. The minimum (Q1-1.5*IQR) = 1 755.6 mg kg–1 and the maximum (Q3-1.5*IQR) = 2 111.10 mg kg–1. Meanwhile, the IQR of fructose in La Boite’s CCF is Q1 = 1 466.7 mg kg–1,

Q2 = 1 533.33 mg kg–1, and Q3 = 1 566.70 mg kg–1. The minimum (Q1-1.5*IQR) = 1 433.30 mg kg–1. IQR of Mengani’s CCF is quite evenly dispersed, while La Boite’s CCF tends to ascend.

The same figure shows median (Q2) of glucose in Mengani’s CCF to be 4 311.10 mg kg–1, which is statistically (P > 0.01) significant when compared to median (Q2) of glucose in La Boite’s

CCF = 3 300.00 mg kg–1. The box plot records glucose content in Mengani’s CCF to be Q1 = 3 911.10 mg kg–1, Q2 = 4 311.10 mg kg–1, and Q3 = 4 422.20 mg kg–1, with minimum (Q1-1.5*IQR) =3 533.30 mg kg–1 and maximum (Q3-1.5*IQR) = 4 533.30 mg kg–1. As for La Boite’s, Q1 = 2 933.30 mg kg–1,

Q2 = 3 300.00 mg kg–1 and Q3 = 3 333.33 mg kg–1 with minimum (Q1-1.5*IQR) = 2 822.20 mg kg–1, and maximum (Q3-1.5*IQR) = 3 366.70. IQR of both Mengani’s CCF and La Boite’s tend to ascend.

Elías (1979) and Murthy and Naidu (2012) stated that reducing sugars in CP was 12.4 %. Sugars are formed in the mesocarp when the exocarp is red. The low rate of reducing sugars in La Boite’s CCF is believed to result from postharvest treatment. A product of Brazil, La Boite’s, should employ dry processing (Padmapriya et al., 2019) where coffee cherry goes directly to drying floors, preventing further metabolism to run in the pulp. On the other hand, Mengani’s is wet processing, where coffee cherry is soaked before peeling under running water. Since the pulp is constantly damp before processing, reducing sugars are formed in the wait.

The problem of wet-processing coffee is categorized as polluting the environment, especially river water and soil (Elida, 2012). Therefore, the authors suggest anaerobic treatment of process water (Setyobudi et al., 2021) in a biogas digester before discharge into rivers. This action is doubly beneficial because, in addition to minimizing pollution, renewable energy from biogas can be used as a coffee bean dryer or CCF.

Conclusions and Recommendations

CP of Arabica Mengani, Bali, Indonesia, is eligible for further processing into CCF, which is beneficial as a source of Fe non-heme. Although Fe content in Mengani’s pulp is relatively low [9.269 mg 100 g–1, ranging (4.717 to 15.686) mg 100 g–1], it is higher than what is contained in S. oleracea (2.7 mg 100 g–1

to 3.9 mg 100 g–1), M. oleifera (7 mg 100 g–1), or

P. dactylifera [(4.06 to 7.06) mg 100 g–1]. Mengani’s CP also contains vitamin C, beta carotene, amino acid, and reducing sugars that act as Fe enhancers. Reducing sugars in it is significantly higher than in La Boite’s CCF. Nevertheless, the oven-drying process of 80 ° C for 15 h needs reviewing, as the enhancers – especially amino acid – tend to get degraded.

Further research should be recommended towards other coffee production centers in Indonesia, particularly in the Java area. Moreover, in vivo studies are suggested to ensure bioavailability on this Fe non-heme content.

Acknowledgments

With sadness, the authors report Yogo Adhi Nugroho, one of the authors of this article, passed away on June 30, 2021, after a fight against COVID-19. We sincerely appreciate his enthusiasm and dedication to the writing of this manuscript. The authors are grateful to Ricky Hendarto Setyobudi and Fitri Ramli, Arabica Coffee Factory at Mengani, Kintamani, Bali – Indonesia, for supplying the CCF as research material. Also, thank you to Yohannes Kohar Whisnu Wardhana, which has brought to Indonesia six CCF packages from La Boite, a store at Manhattan, 724 11th Avenue New York, NY 10019.

Novelty Statement

A product of the circular economy and environmentally friendly, Coffee Cherry Flour (CCF) has been around American and European markets since 2014. Reducing coffee production costs, minimizing pulp pollutants, and creating functional food are the reasons behind its invention. As the fourth biggest coffee producer globally, it is only proper for Indonesia to research and develops CCF as a beneficial commodity. This study has gained data that vitamin C in CCF acts as a Fe non-heme enhancer and that Mengani coffee pulp is able to serve as a Fe non-heme source. Vitamin C levels are not listed in the leaflets of several CCF manufacturers.

Author Contribution

RHS: Conceptualized and designed the study, elaborated the intellectual content, performed literature search, data acquisition, data analysis, manuscript format, manuscript preparation and manuscript revision.

EY: Elaborated the intellectual content, performed literature search, manuscript review, check and re-check similarity (Turnitin), and Premium Grammarly.

YAN, MSS and SKW: Statistic analysis, data conversion, carried out experimental studies, performed the literature search, and manuscript preparation.

WW, LZ and DD: Elaborated the intellectual content, research supervision, performed the literature search, and manuscript review.

EAS and MM: Elaborated the intellectual content, performed the literature search, and manuscript review.

MFMA and MIM: Performed the literature search, manuscript review, and guarantor.

DY, DR, PGA and SM: Performed the literature search and manuscript review.

RKM and HS: Figure, performed the literature search, and manuscript review.

All authors read and approved the final manuscript

Conflict of interest

The authors have declared no conflict of interest.

References

Acosta, L.L. 2019. Colombia coffee federation calls for $2 floor for prices. Reuters July, 3, 2019. (https://www.reuters.com/article/colombia-coffee/colombia-coffee-federation-calls-for-2-floor-for-prices-idUSL8N2435VW)

Agusmayanti, R., A. Farich and A. Anggraini. 2020. Vitamin C can increase hemoglobin levels in anemic pregnant women. Jurnal Kebidanan Malahayati. 6(3): 342–348. https://doi.org/10.33024/jkm.v6i3.1731

Alakali, J.S., C.T. Kucha and I.A. Rabiu. 2015. Effect of drying temperature on the nutritional quality of Moringa oleifera leaves Afr. J. Food Sci.., 9: 395-399. https://doi.org/10.5897/AJFS2014.1145

Anna, B., A. Haryanto, U. Hasanudin, D.A. Iryani, M. Telaumbanua and D. Herák. 2019. Sustainable management of coffee fruit waste biomass in ecological farming systems at West Lampung, Indonesia. IOP Conf. Ser. Earth Environ. Sci., 345(012007): 1–10. https://doi.org/10.1088/1755-1315/345/1/012007

AOAC. 1995. Official methods of analysis 16th Ed. Association of Official Analytical Chemists. Washington DC, USA.

Ari, Y. 2014. Micromineral nutrition and health. Unnes Press, Semarang, Indonesia.

Asfimanto, W.A., T. Nurlambang and T. Waryono. 2013. Effect of physical condition and cultivation on the quality coffee in Kintamani and Gayo. Fakultas FMIPA, Universitas Indonesia, Jakarta. (https://nanopdf.com/queue/pengaruh-kondisi-fisik-dan-budidaya-terhadap-kualitas-kopi-di_pdf?queue_id=-1&x=1644534641&z=MTU4LjE0MC4xNjMuNDU=).

Avinash, S.N., C.A. Srinivasamurthy, S. Bhaskar and N.B. Prakash. 2017 Characterization, extraction and foliar spray of fortified humic acid on quality of capsicum. Int. J. Curr. Microbiol. Appl. Sci., 6(10): 2265‒2272. https://doi.org/10.20546/ijcmas.2017.610.268

Bukhari, A., H. Firdaus, M. Rahmawati, S.N. Sheryl and M.C. Prisilia. 2020. Non-nutritional and disease-related anemia in Indonesia: A systematic review. Asia Pac. J. Clin. Nutr. (supplement). 29: S.41–S.54.

CGTN America. 2014. Coffee flour: Turning waste into a valuable ingredient. (https://www.youtube.com/watch?v=SVbtroYpKVc).

Christides, T. and P. Sharp. 2013. Sugars increase non-heme iron bioavailability in human epithelial intestinal and liver cells. PLoS ONE. 8(12): e83031. https://doi.org/10.1371/journal.pone.0083031

Coffee Cherry Co. 2021. Nutrition. (https://coffeecherryco.com/nutrition/)

Coffee Flour BJ - Baker Josef’s. 2021. Nutrition facts. (https://www.eatthismuch.com/food/nutrition/coffee-flour,1582010/)

Conner, T.A., C. McQuade, J. Olp and A.B. Pai. 2012. Effect of intravenous vitamin C on cytokine activation and oxidative stress in end-stage renal disease patients receiving intravenous iron sucrose. Biometals, 25: 961–969. https://doi.org/10.1007/s10534-012-9562-6

Damat, D., R. Angriani, R.H. Setyobudi and P. Soni. 2019. Dietary fiber and antioxidant activity of gluten-free cookies with coffee cherry flour addition. Coffee Sci., 14(4): 493–500.

Damayanti, S., P.D. Swamilaksita and D. Angkasa. 2016. The effect of iron, vitamin A, vitamin B12, and vitamin C intake on hemoglobin levels in vegetarian adolescents. Jurnal Gizi Klinik Indonesia, 13(2):67–74.

Das, A. and M. Das. 2020. Impact of nutritional status and anemia on COVID-19: Is it a public health concern? Evidence from National Family Health Survey-4 (2015–2016), India. Public Health. 185: 93–94.

Deborah, T. 2021. See how coffee “cherries” make a high-antioxidant flour. Farmers Almanac. (https://www.farmersalmanac.com/what-is-coffee-flour-28035)

Dheny, R. and T. Umarianti. 2017. Effectiveness give of bayam extract againts enhancement levels hemoglobin on mother pregnant with mild anemia. Jurnal Kebidanan, 9(2): 165–174. https://doi.org/10.35872/jurkeb.v9i02.318

DKBM Indonesia. 2017. List of Indonesian food composition. (http://staffnew.uny.ac.id/upload/132318122/pendidikan/DKBMIndonesia).

Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem., 28(3): 350–356.

Ebba, B. 2015. A nutritional analysis on the by product coffee husk and its potential utilization in food production – A literature study. Bachelor Thesis. Faculty of Natural Resources and Agricultural Sciences Department of Food Science, Swedish University of Agricultural Sciences, Sweden. https://stud.epsilon.slu.se/8486/7/bondesson_e_150922.pdf?fbclid=IwAR3NoI1K-AlcyIOQiy0uT-VMd1iBEc4nxvyQN93HUss7laI0fwEfM0lpqUU.

Egbuna, C. 2015. Moringa oleifera the mother’s best friend. Int. J. Nutr. Food Sci., 4(6): 624–630. https://doi.org/10.11648/j.ijnfs.20150406.14

Elba, C., R.B. Ana and G. Eva. 2017. Coffee berry processing by-product valorization: Coffee parchment as a potential fiber source to enrich bakery goods. J. Food Nutr. Popul. Health., 1(2):1–7.

El-Hawary, M.F.S., F.A. El-Shobaki, T. Kholeif, R. Sakr and M. El-Bassoussy. 1975. The absorption of iron, with or without supplements of single amino acids and of ascorbic acid, in healthy and Fe-deficient children. Br. J. Nutr., 33(3): 351–355.

Elías, L. 1979. Chemical composition of coffee-berry by-products. p. 11–16. In: Braham J. and R. Bressani (eds). Coffee pulp: Composition, technology, and utilization. Institute of Nutrition of Central America and Panama, Ottawa, Canada.

Elida, N. 2012. Process design in robusta coffee agroindustry using modified wet processing technology based on clean production. PhD Dissertation. Sekolah Pascasarjana, Institut Pertanian Bogor, Indonesia. https://repository.ipb.ac.id/handle/123456789/55728?show=full

Ellie S.S., K. Sun, S. de Pee, K. Kraemer, R. Jee-Hyun, R. Moench-Pfanner, M. Sari, M.W. Bloem and R.D. Semba. 2012. Relationship of maternal knowledge of anemia with maternal and child anemia and health-related behaviors targeted at anemia among families in Indonesia. Matern. Child Health J., 16: 1913–1925. https://doi.org/10.1007/s10995-011-0938-y

Emily, L. 2015. What is coffee flour? TED Blog - Conferences. (https://blog.ted.com/what-is-coffee-flour/).

FDA. 2020. (US Food and Drug Administration). How to understand and use the nutrition facts label. (https://www.fda.gov/food/new-nutrition-facts-label/how-understand-and-use-nutrition-facts-label#top)

Fernanda, B.M., J.M. Oliveira, J. Stefanello, L.A. Luzia and P.H.C. Rondó. 2013. The influence of vitamin A supplementation on iron status. Nutrients, 5: 4399–4413. https://doi.org/10.3390/nu5114399

Ferry A.S. 2018. Indonesian coffee won gourmet product awards in Paris, France. Times Indonesia (https://www.timesindonesia.co.id/read/news/188401/indonesian-coffee-won-gourmet-product-awards-in-paris-France).

Global Holdings, Inc. 2015. Coffee flour the new global impact food. An innovation overview. Clean Label Conference, March 31 - April 1, 2015 Itasca, Illinois, USA.

Grisel, C., U. Pal and S. Cebada 2014. Enhanced biogas production from coffee pulp through deligninocellulosic photocatalytic pretreatment. Energy Sci. Eng., 4:2(4): 177–187. https://doi.org/10.1002/ese3.44

Hasibuan, S., B.K. Djatmo, K.H. Nitimulyo and E. Hanudin. 2014. Abundance of natural feed on inceptisols pond bottom soil by marling it with ultisols. Jurnal Dinamika Pertanian, 29(1): 97–106.

Hermosa, B., V.A. Duarte and R.F. Schwan. 2014. Utilization of coffee by-products obtained from semi-washed process for production of value-added compounds. Bioresour. Technol., 166: 142–150. https://doi.org/ 10.1016/j.biortech.2014.05.031.

Ika, A., R. Nurjanah, A.A. Suwandi, Suryani, T. Hidayat. 2015. Amino acids, fatty acids, cholesterol and mineral composition on eel (Monopterus albus) due to deep frying. Depik, 4(1): 49-57. http://dx.doi.org/10.13170/depik.1.1.2365.

Indriani, L., C. Zaddana, N.M. Nurdin and J.S.M. Sitinjak. 2019. The effect of nutrition education and moringa leaf powder capsules (Moringa oleifera L.) on the increase in hemoglobin levels of adolescent girls at Pakuan university. Media Pharmaceutica Indonesiana, 2(4): 200–207. https://doi.org/10.24123/mpi.v2i4.2109.

Iriondo-DeHond, A., M. Iriondo-DeHond and M. Dolores del Castillo. 2020. Applications of compounds from coffee processing by-products. Biomolecules, 10 (1219): 1–20. http://dx.doi.org/10.3390/biom10091219.

James, C. and F. Deswarte. 2015. The biorefinery concept: An integrated approach. p. 1–29. In: James C. and F. Deswarte (eds), Introduction to chemicals from biomass. Wiley Online Library. John Wiley & Sons, Ltd., New Jersey, USA. https://doi.org/10.1002/9781118714478.ch1

Jumadi. 2020. The effect of iron biofortified maize on haemoglobin and erythrocyte level of Wistar rat anemia. Disertation Universitas Hasanudin, Makasar, Indonesia. (http://digilib.unhas.ac.id/uploaded_files/temporary/DigitalCollection/IyY==.pdf)

Kang, T.H., W. Setyo, W. Irawaty and F.E. Soetaredjo. 2007. Effect of temperature and heating time on the content of vitamins A and C in the process of making tomato paste. Widya Teknik, 6(2):111–120. https://doi.org/10.33508/wt.v6i2.1237

Kayhanian, M. and G. Tchobanoglous. 2016. Energy recovery by anaerobic digestion process. p 1529‒1580. In: Goswani, D.Y. and F. Kreith (eds). Handbook of Energy Efficiency and Renewable Energy, Second Edition, Section IV-48. CRC Press. Boca Raton, Florida, USA.

Kemenkes, R.I., Kementrian Kesehatan Republik Indonesia. 2018. Indonesian food composition data. (https://www.panganku.org/en-EN/beranda).

Kevon, R.Z.G., C. Knudson, J. Avelino, C.M. Bacon, G. Leclerc, M.C. Aime and D.P. Bebber. 2021. Epidemics and the future of coffee production. Proc. Natl. Acad. Sci., 118 (27): e2023212118. https://doi.org/10.1073/pnas.2023212118

Kurniawan. A.M. 2020. A brief overview between coffee and COVID-19 pandemic. Zenodo, Article 5199509:1–7. https://doi.org/10.5281/zenodo.5199509

Kurniawati, A.D. 2016. Kinetics of thermal degradation of carotenoids in the evaporation process for the manufacture of tomato puree concentrate (Lycopersicum esculentum Mill.). Undergraduaded Thesis, Universitas Brawijaya, Malang, Indonesia. http://repository.ub.ac.id/150773/

Machmudah S. and M. Goto. 2013. Methods for extraction and analysis of carotenoids. In: Ramawat K. and JM. Mérillon (eds). Natural Products. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22144-6_145

Mahendra, D.S., Tamrin and B. Lanya. 2014. The performance test of mechanical rack grain dryer with three environmental conditions. Jurnal Teknik Pertanian Lampung, 3(1): 91–102.

Marín-Tello, C., L. Zelada-Castillo, A. Vásquez-Arqueros, A. Vieira and R. Siche. 2020. Coffee pulp: An industrial by-product with uses in agriculture, nutrition and biotechnology. Rev. Agric. Sci., 8: 323–342. https://doi.org/10.7831/ras.8.0_323

Meiliana, M. and N. Roekisti and S. Endang. 2014. The effect of processing of cassava (Manihot esculenta Crantz.) leaves with various treatments on -carotene levels. Indonesian J. Human Nutr., 1 (1):23–34.

Melkayo, G., Y.B. Tola and A. Sualeh. 2016. Extraction and determination of total polyphenols and antioxidant capacity of red coffee (Coffea arabica L.) pulp of wet processing plants. Chem. Biol. Technol. Agric., 3(25): 1–6. https://doi.org/10.1186/s40538-016-0077-1

Milman, N. 2011. Anemia-still a major health problem in many parts of the world!. Ann. Hematol., 90: 369–377. https://doi.org/10.1007/s00277-010-1144-5.

Mindarti, S., L. Zalizar, Damat, E.A. Saati and S. Fajriani. 2020. Characterization of fiber fraction, physical and chemical properties of coffee flour (Coffea sp.) as functional foodstuff for diabetes mellitus patient. IOP Conf. Ser. Earth Environ. Sci., 462 (012017):1–10. https://doi.org/10.1088/1755-1315/462/1/012017

Moreno, J., S. Cozzano, A.M. Pérez, P. Arcia and A. Curutchet. 2019. Coffee pulp waste as a functional ingredient: Effect on salty cookies quality. J. Food Nutr. Res., 7(9): 632–638. https://doi.org/10.12691/jfnr-7-9-2

Murphy, H. 2019. Colombia losing coffee crop area due to low prices. Reuters Commodities. May 9, 2019. (https://www.reuters.com/article/us-colombia-coffee/colombia-losing-coffee-crop-area-due-to-low-prices-idUSKCN1SE2HK)

Murthy, P.S. and M.M. Naidu. 2012. Sustainable management of coffee industry by-products and value addition—A review. Resour. Conserv. Recycl., 6: 45–58. https://doi.org/10.1016/j.resconrec.2012.06.005

Nadeem, M., T.M. Qureshi, I. Ugulu, M.N. Riaz, Q.U. Ain, Z.I. Khan, K. Ahmad, A. Ashfaq, H. Bashir and Y. Dogan. 2019. Mineral, vitamin and phenolic contents and sugar profiles of some prominent date palm (Phoenix dactylifera) varieties of Pakistan. Pak. J. Bot., 51(1): 171–178. https://doi.org/10.30848/PJB2019-1(14).

Nandini, R. and B.H. Narendra. 2012. Critical land characteristics of former eruption of Batur mount in Bangli district, Bali. Jurnal Penelitian Hutan dan Konservasi Alam, 9: 3:199–211.

Nasution, R.S., A. Siti and H.H. Pratiwi. 2021. Consumption of red spinach juice can increase hemoglobin levels in pregnant women in the first trimester. Jurnal Kebidanan Malahayati, 7(2): 215–221. https://doi.org/10.33024/jkm.v7i2.3420.

Nizar A. 2018. Protection of geographical indications within the scope of improvement of regional economy. De Jure 18(4): 525–542. http://dx.doi.org/10.30641/dejure.2018.V18.525-542

Nurbadriyah, W.D. 2019. Iron deficiency anemia. Deepublish publisher, Yogyakarta. Indonesia.

Nur, A.F. and N. Maulany. 2021. Consumption of dates to increase hemoglobin levels in third trimester pregnant women with anemia disorders. Majalah Kesehatan Indonesia. 2 (2): 49–54. https://doi.org/10.47679/makein.202136.

Nurul, A., C. Epriyani, A. Kurnia, K. Ramadhan, S.G. Hidayat and A. Apriyantono. 2022. Kintamani Bali arabica coffee profile. In: Nurul, A. and A. Apriyantono (eds). AE Publishing, Malang, Indonesia.

Oestreich-Janzen, S.H. 2013. Chemistry of coffee. p. 1085–1113. In: 3.25. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. Science Direct – Elsevier. https://doi.org/10.1016/B978-0-12-409547-2.02786-4

Padmapriya, R., J.A. Tharian and T. Thirunalasundari. 2019. Coffee waste management-An overview. Int. J. Curr. Res., 33(1):9–16.

Petek, E.T., S.A. Gómez-Ochoa, E. Llanaj, P.F. Raguindin, L.Z. Rojas, Z.M. Roa-Díaz, D. Salvador Jr, D. Groothof, B. Minder, D. Kopp-Heim, W.E. Hautz, M.F. Eisenga, O.H. Franco, M. Glisic and T. Muk. 2020. Anemia and iron metabolism in COVID-19: A systematic review and meta-analysis. Eur. J. Epidemiol., 35: 763–773. https://doi.org/10.1007/s10654-020-00678-5

Pratiwi, W. 2015. Honey to prevent iron deficiency anemia in pregnancy. Jurnal Majority 4 (3): 90–95.

Priyo, S., Y. Heriyanto, I. Priyadi, L.F Putri and O. Rilkiyanti. 2020. Analysis and synchronization of food composition table application nutri survey version Indonesia. Jurnal Nutrisia, 22(1): 39– 45. https://doi.org/10.29238/jnutri.v22i1.201

Pushpa, S.M. and M. Naidu. 2012. Sustainable management of coffee industry by-products and value addition – a review. Resour. Conserv. Recycl., 66:45–58. https://doi.org/10.1016/j.resconrec.2012.06.005

Pushpa S.M., M.R. Manjunatha, G. Sulochannama and M. Naidu. 2012. Extraction, characterization and bioactivity of coffee anthocyanins. Eur. J. Biol. Sci., 4 (1): 13–19. https://doi.org/10.5829/idosi.ejbs.2012.4.1.6149

Qiu, K., C.F. Qin, M. Luo, X. Zhang, W.J. Sun, N. Jiao, Li de F. and J.D. Yin. 2016. Protein restriction with amino acid-balanced diets shrinks circulating pool size of amino acid by decreasing expression of specific transporters in the small intestine. PLoS One. 11(9): e0162475. https://doi.org/10.1371/journal.pone.0162475

Rachel, C. 2019. Well hello, coffee cherry flour. Foods Finds. (https://www.foodscapefinds.com/content/2019/3/9/well-hello-coffee-cherry-flour)

Rahardjo, B., B.M.B. Akbar, Y. Iskandar and A. Shalehah. 2020. Analysis and strategy for improving Indonesian coffee competitiveness in the international market. Bisnis dan Manajemen. 12:154–167. https://doi.org/10.26740/bisma.v12n2.p154-167.

Ramesh, D., G.R. Manjunatha, P. Mishra, A.K. Tailor and B.S. Dhekale. 2019. Design experiments in the basic of statistics in agricultural sciences. Apple Academic Press Inc. Canada.

Rania M.A.M., A.S.M. Fageer, M.M. Eltayeb and I.A.M. Ahmed. 2014. Chemical composition, antioxidant capacity, and mineral extractability of Sudanese date palm (Phoenix dactylifera L.) fruits. Food Sci. Nutr., 2(5): 478–489.

Resti, D.S., A.N. Usman, Y. Widaningsih, N. Jafar, M. Ahmad, S. Ramadhani and A. Dirpane. 2021. Date palm (Phoenix dactylifera) consumption as a nutrition source for mild anemia. Gac Sanit. 35(S2): S271–S274. https://doi.org/10.1016/j.gaceta.2021.10.032

Rizki, A., Nazaruddin and D. Handito. 2018. The effect of boiling time on vitamin C, antioxidant activity, and sensory properties of Singapore cherry (Muntingia calabura L.) syrup. Pro Food. 4(1): 289–297. https://doi.org/10.29303/profood.v4i1.77

Rodriguez-Amaya, D.B and M. Kimura. 2004. Harvestplus handbook for carotenoid analysis. Harvestplus technical monograph 2. International Food Policy Research Institute (IFPRI) and International Center for Tropical Agriculture (CIAT). Washington, DC and Cali.

Rosas-Sánchez, G.A., Z.J. Hernández-Estrada, M.L. Suárez-Quiroz, O. González-Ríos and P. Rayas-Duarte. 2021. Coffee cherry pulp by-product as a potential fiber source for bread production: A fundamental and empirical rheological approach. Foods, 10(4): 742: 1–12. https://doi.org/10.3390/foods10040742

Setyobudi, R.H., L. Zalizar, S.K. Wahono, W. Widodo, A. Wahyudi, M. Mel, B. Prabowo, Y. Jani, Y.A. Nugroho, T. Liwang and A. Zaebudin. 2019. Prospect of Fe non-heme on coffee flour made from solid coffee waste: Mini review. IOP Conf. Ser. Earth Environ. Sci., 293 (012035):1–24. https://doi.org/10.1088/1755-1315/293/1/012035

Setyobudi, R.H., S.K. Wahono, P.G. Adinurani, A. Wahyudi, W. Widodo, M. Mel, Y.A Nugroho, B. Prabowo and T. Liwang. 2018. Characterisation of arabica coffee pulp - hay from Kintamani - Bali as prospective biogas feedstocks. MATEC Web Conf. 164 (01039): 1–13. https://doi.org/10.1051/matecconf/201816401039

Setyobudi, R.H., E. Yandri, M.F.M Atoum, S.M. Nur, I. Zekker, R. Idroes, T.E. Tallei, P.G. Adinurani, Z. Vincēviča-Gaile, W. Widodo, L. Zalizar, N. Van Minh, H. Susanto, R.K. Mahaswa, Y.A. Nugroho, S.K. Wahono and Z. Zahriah. 2021. Healthy-smart concept as standard design of kitchen waste biogas digester for urban households. Jordan J. Biol. Sci., 14(3): 613–620. https://doi.org/10.54319/jjbs/140331

Shahbandeh, M. 2020. Global coffee consumption 2012/13-2018/19. (https://www.statista.com/statistics/292595/global-coffee-consumption)

Stéphane, O. and D. Thomas. 2009. Biorefinery: Toward an industrial metabolism. Biochimie 91(6): 659-664. https://doi.org/10.1016/j.biochi.2009.03.015

Sukatiningsih. 2011. The encapsulation of antioxidant extract of coffee husk using a combination of gum arabic and tapioca is oxidized as a capsule material. Research Report. University of Jember. http://repository.unej.ac.id/bitstream/handle789/58861/Sukatiningsih_unggulan_boptn_129.pdf?sequence=1

Sukrisno, W. 2013. The potential and technology of diversifying coffee waste into quality and value-added products. Review Penelitian Kopi dan Kakao 1 (1): 63–80.

Sultana, P., D. Easmin, A. Sheikh, M. Biswas, S.C.D. Sharma, Md. G.S. Jahan, Md. A. Islam, N. Roy and M.S. Shovon. 2015. Nutritional analysis of date fruits (Phoenix dactylifera L.) in perspective of Bangladesh. Am. J. Life Sci., 3(4) 274–278. https://doi.org/10.11648/j.ajls.20150304.14

Tri, H. and S. Sunarsih. 2021. Consumption of Moringa leaf extract in increasing hemoglobin levels in pregnant women. Malahayati Nursing J., 3(1):101–107. https://doi.org/10.33024/manuju.v3i1.3231

Usamah, J., D. Wirnas, Trikoesoemaningtyas and H. Purnamawati. 2019. Effect of high-temperature conditions to rice growing and yield. J. Agron. Indonesia 47(3):248–254. https://dx.doi.org/10.24831/jai.v47i3.24892

USDA. 2018 (United States Department of Agriculture, Agricultural Research Service). National nutrient database for standard reference legacy release (https://ndb.nal.usda.gov/ndb/foods/show/11457)

Van Der Schaar Investments B.V. 2019. A cup of Indonesian coffee; production, export and consumption of Indonesian coffee. In: Indonesia investment 2019, A new cabinet arrives report October 2019. Deft and Jakarta. pp. 47–58.

Wich, D. 2015. Coffee flour: New potential ingredient? https://raypeatforum.com/community/threads/coffee-flour-new-potential-ingredient.7194

WIPO – PCT. 2014. WO2014/158267A1. The coffee cherry flour compositions and methods for their preparation. World Intelectual Property Organization International Bureau – Patent Cooperation Treaty. (https://patents.google.com/patent/WO2014158267A1/en)

Xiandeng, H., R.S. Amais, B.T. Jones and G.L. Donati. 2016. Inductively coupled plasma optical emission spectrometry. Wiley Online Library. https://doi.org/10.1002/9780470027318.a5110.pub3

Zmrhal, Z.J., J.G. Heathcote and R.J. Washington. 1975. Chapter 32 Amino acids. J. Chromatograph. Library, 3:665–711. https://doi.org/10.1016/S0301-4770(08)61196-3

Zupancic, G.D. and V. Grilc. 2012. Anaerobic treatment and biogas production from organic waste. p. 3–28. In: Sunil, K. and A. Bharti (eds). Chapter 1. Management of Organic Waste. IntechOpen Limited, London, UK. https://doi.org/10.5772/32756