Anticancer Activity Induced by a Rare Ginsenoside Compound K in Human Neuroblastoma
Anticancer Activity Induced by a Rare Ginsenoside Compound K in Human Neuroblastoma
Seun Eui Kim1,2*, Myoung-Hoon Lee1, Hye Myoung Jang2, Garam Park2, Wan-Taek Im3, Gwang Joo Jeon2,4*
Cell viability of CK-treated cells and IC50 concentration of CK against neuroblastoma cells. (a) SK-N-MC cells were treated with 0, 20, 40, 80, 100 µM of CK and cultured at 37°C, 5% CO2 incubator for 48 h, and then the OD450 of the cells was measured (Mean ± SE, * p < 0.01). (b) Cell viability of each treatment group was expressed as a percentage based on the control group. The IC50 concentration of CK for 50% survival of neuroblastoma was determined by plotting a linear regression curve.
Scratch wound healing assay to confirm the effect of CK to inhibit migration of neuroblastoma cells. (Left) SK-N-MC cells were treated with 0, 20, 40, and 60 µM of CK for 48 h and images were recorded at 0 h and 48 h after scratching. (Right) The wound coverage % was determined by the aera of cells migrating towards the scratched area over time using ImageJ TM software (Mean ± SE, * p < 0.05).
Expression of Apoptosis-regulatory genes after treatment with CK Relative expression of genes was determined by qPCR in SK-N-MC cells treated with or without CK of the concentration of IC50 for 24 h. Result means (±SE) of three biological replicates. All protein expressions were significantly different (at p=0.05) except Caspase 12.
