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Antibacterial Effect of Bay Leaf (Laurusnabilis) Aqueous Extract and its Nano-Emulsion on Some Pathogenic Bacteria

Antibacterial Effect of Bay Leaf (Laurusnabilis) Aqueous Extract and its Nano-Emulsion on Some Pathogenic Bacteria

Suhair Sh. Al-Siraj1, Jihan M. Badr2 and Dalia M.A. El-Masry3*

1Biology Department, Faculty of Science, Mustansiriyah University, Bagdad, Iraq; 2Poultry Diseases Department,  Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza, Egypt; 3Nanotechnology Research unit, Animal Health Research institute, Agricultural Research center (ARC), Giza, Egypt.
 
*Correspondence | Dalia M.A. EL-MASRY, Nanotechnology Research Unit, Animal Health Research Institute, ARC, Giza, Egypt; Email: [email protected]

Table 1:
Primers sequences, target genes, amplicon sizes and cycling conditions.
Table 2:

Primers sequences, target genes and cycling conditions for SYBR green rt-PCR.

Figure 1:

HRTEM of Bay leaf (Laurus nobilis) nanoparticles showed nano sphere form, no aggregation and size (33.15) nm with Mag. 5000× to 123000× and 40000× to 91100× (Central lab. in NRC).

Figure 3:
Antibacterial activity of Bay leaves (laurus nobilis) Aqueous extracts and nanoemulsion against E. coli and Pseudomonas aeruginosa
1=Bay leaf (laurus nobilis) Aqueous extract , 2= Bay leaf (laurus nobilis) nano emulsion
 - = negative control, + = positive control.
Figure 2:
Antibacterial activity of Bay leaves (laurus nobilis) Aqueous extracts and nano emulsion against S. aureus
1-Bay leaf (laurus nobilis) Aqueous extract , 2- Bay leaf (laurus nobilis) nano emulsion, - = negative control, + = positive control.
Figure 4:

Results of PCR analysis of identification of resistance and virulence genes of S. aureus and E. coli.

Figure 5:

Relative mecA, coa, and blaZgenes expression of S. aureus before and after treatment with laurus nobilis nanoemulsion extract .

Figure 6:

Relative Stx2, iss, floR, Mcr1genes expression of E. coli before and after treatment with laurus nobilis nanoemulsion extract. 

Advances in Animal and Veterinary Sciences

December

Vol. 12, Iss. 12, pp. 2301-2563

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