An Optional Low-Cost Method of Extracting Environmental DNA of Macro-Organisms from Filter Membranes in Large Scale eDNA Surveys
An Optional Low-Cost Method of Extracting Environmental DNA of Macro-Organisms from Filter Membranes in Large Scale eDNA Surveys
Zhi Chen1, Toshifumi Minamoto2, Longshan Lin3 and Tianxiang Gao4,*
Fig. 1.
Water sampling sites of this study. From left to right, the map was gradually enlarged.
Fig. 2.
Sediment concentration of all water samples.
Fig. 3.
The DNA yield (copies/300 ml) of Sepiella japonica extracted from aquaculture water (indoors water sample). There was no difference in eDNA yield between the DNeasy Blood and Tissue Kit and AxyPrep DNA Gel Extraction Kit groups (p=0.061).
Fig. 4.
Agarose gel electrophoresis results of 4 field water samples. M, DNA marker.
February 2021
Vol. 53, Iss. 1, Pages 1-400
