Research Article

Aloe Vera Gel Coating Retains Persimmon Fruit (Diospyros kaki) Quality during Storage at Room Temperature

Abdul Jalal, Abdur Rab* and Naveed Ahmad

Department of Horticulture, Faculty of Crop Production Sciences, University of Agriculture, Peshawar, Pakistan.

Received | March 24, 2019; Accepted | August 23, 2020; Published | October 17, 2020

*Correspondence | Abdur Rab, Department of Horticulture, Faculty of Crop Production Sciences, University of Agriculture, Peshawar, Pakistan; Email: [email protected]

Citation | Jalal, A., A. Rab and N. Ahmad. 2020. Aloe vera gel coating retains persimmon fruit (Diospyros kaki) quality during storage at room temperature. Sarhad Journal of Agriculture, 36(4): 1094-1102.

DOI | http://dx.doi.org/10.17582/journal.sja/2020/36.4.1094.1102

Keywords | Aloe vera, Ascorbic acid, Persimmon, Quality, TSS

Introduction

Persimmon (Diospyros kaki), belonging to family Ebenaceae, is native to oriental Asia (Baltacioglu and Artik, 2013). The persimmon fruit is a good source of ascorbic acids, sugars, carotenoids, organic acids, vitamins, minerals, phenolic compounds and dietary fiber (Del-Bubba et al., 2009; Veberic et al., 2010). Being rich in ascorbic acid and phenolic compounds, the persimmon fruit may protect against oxidative stress (Scalbert and Williamson, 2000; Suzuki et al., 2005). The Persimmon fruit rapidly deteriorates during storage mainly due moisture loss and pathological disorders (Gorinstein et al., 2001). Pre and postharvest infections causing decay and moisture loss are the major causes of quality deterioration during storage (Terry and Joyce, 2004; Misir et al., 2014). Thus, delaying softening and disease control are the primary techniques to extend the storage life of persimmon fruit (Khademi, 2012).

Attempts have been made to increase the storage life of persimmon fruit by the application of chemicals (Zheng et al., 2006; Bagheri et al., 2015). However, the use of chemicals to increase the postharvest life of fruits has limited scope due to potential risks to human health (Asghari et al., 2013). Recently edible coatings have developed for preserving the horticultural commodities and improve the appearance of the fruit (Mandal et al., 2009). Edible coatings prevent moisture loss and reduce the rate of respiration during storage, thus, delays senescence (Serrano et al., 2006). Some edible coatings are added with anti-microbial compounds to prevent disease incidence and retain visual quality (Cha and Chinnan, 2004).

Aloe vera gel has long been used for medicinal purposes (Valverde et al., 2005). Aloe vera gel has two major components; the liquid yellow latex and clear gel extract from the parenchyma tissue of the leaves (Ni et al., 2004). Aloe vera gel is mainly used in industries for making cosmetics and can be used for healing of wounds, burns and scars (Serrano et al., 2006). Due to its waxy nature and anti-microbial activities, Aloe vera gel can also be used as bio preservative to extend the storage life of horticultural commodities (Martinez-Romero et al., 2006). Aloe vera gel, as a coating material, suppressed respiration, decreases fruit softening, reduce moisture loss, delays senescence, reduced microorganism proliferation and delays oxidative browning in sweet cherries, table grapes, nectarines and papaya (Ahmad et al., 2009; Marpudi et al., 2013). Keeping in view the potential benefits of Aloe vera Gel, as bio-preservative and edible coating material, the current research was conducted to evaluate the influence of different concentrations of Aloe vera Gel on the ripening and quality losses of persimmon fruit during storage at room temperature.

 

Materials and Methods

The influence of different concentrations of Aloe vera gel, as a bio preservative, on the storage life and quality of persimmon fruit was investigated by treating persimmon fruits with 0, 10, 20, 30 and 40% Alove vera gel and storage for 0, 7, 14, 21, 28 and 35 days at room temperature. Persimmon fruits (Var. Seedless Persimmon; PCNA type: Pollination constant non astringent) were harvested from Horticulture Farm of the University of Agriculture, Peshawar, during the month of October, 2014 at Physiological (pinkish stage of) maturity. The fruits were carefully shifted to the laboratory in wooden boxes. Physically sound and healthy fruits were separated and washed with running tap water followed by rinsing with distilled water. The surface moisture was removed from the fruits with a gentle blower. Total number of treatments were 60 and the number of fruits per treatment was 8 having the size of 129±3 cm3.

Aloe vera gel preparation

Fully matured leaves of Aloe vera plants were harvested early in the morning and shifted to the postharvest Laboratory. The Aloe vera leaves were positioned in upright orientation, in an iron stand for 30 minutes to remove all the yellow latex. The vertical corners of leaves were removed and then cut into slices along with the green rind tissue. The transparent gel was carefully separated from the rind tissue, blended in a blender and filtered through a soft netted cloth to remove any particle of the rind and fiber tissue. The Aloe vera juice was collected in a beaker wrapped with light protecting paper (Adetunji et al., 2012). The Aloe vera juice was stored in dark jar and kept at refrigerated temperature (4 °C) to prevent spoilage (Mohebbi et al., 2011). This gel was then heated in oven for 45 minutes at 70 °C and then cooled to the ambient temperature adding 2 g.L-1 of ascorbic acid and warmed to 23°C for 15 minutes adding 4.5 g. L-1 of citric acid for maintaining its pH at 4 (Marpudi et al., 2013). Different concentrations of Aloe vera gel were prepared by adding gel to distilled water.

Treatment of fruits

The persimmon fruit were carefully dipped in different concentrations (0, 10, 20, 30 and 40%) of Aloe vera gel for 5 minutes and allowed to stay on clean paper sheet for 15 minutes. The fruits were then stored for 0, 7, 14, 21, 28 and 35 days at room temperature (26±2 °C). The fruits were evaluated for different quality parameters at the end of each storage interval.

Evaluation of quality parameters

Fruit firmness was measured with handhold penetrometer (Effegi Fruit Firmness Tester- FT 327). A small portion fruit skin, at the equatorial region of the fruit, was peeled and the penetrometer probe was gently inserted into the peeled surface to the desired depth (as per manufacturer’s protocol) and the fruit firmness was recorded.

Decay incidence was recorded by storing a separate lot of 50 fruit from each treatment and replication. The number of fruits with disease or decay symptoms were calculated at each storage interval and converted to percent decay incidence using the following formula.

The organoleptic quality evaluation was carried out by panel of 5 judges and scored at 1-9 points’ hedonic scale (Raza et al., 2013). The fruit samples, treated with various Aloe gel concentrations were evaluated for as organoleptic quality attributes (color and taste) at each storage interval. The score allocated was averaged to represent the organoleptic quality of the persimmon fruit.

The Total Soluble Solids (TSS) was estimated with a handhold refractometer (Crisosto-MT-032) at each storage interval. For this purpose, the fruit juice was extracted from the fruits and a few drops were added to the prism of refractometer and slid was placed properly and TSS content was recorded. The prism was washed with distilled water between successive readings.

The titratable acidity of the fruits was determined at each storage interval by titration of the fruit juice sample against 0.1 N NaOH solution (AOAC, 1990). For this purpose, 10 ml sample of fruit juice was taken in a graduated cylinder and the volume was made to 100 ml with distillated water. Titration of 10 ml from the 100 ml sample was done against 0.1N NaOH solution using liquid phenopthaline as an indicator till the persistence of light pink color. Three readings were taken for each treatment and then the means of these reading were calculated. The calculation of percent acidity was done by the given formula.

Where;

T= NaOH used (ml); L= Sample taken in wt. for dilution; M= Diluted sample taken for titration in ml.

The ascorbic acid of the fruits was estimated for all the treatment at each storage interval by titrating the fruit juice sample against dye standard solution AOAC (1990). The dye solution was prepared by taking 50 mg 2, 6 Dichlorophenol Indophenol dye and 42 mg Sodium Bicarbonate (NaHCO2) in a beaker containing distilled water. It was heated for 30 minutes at a magnetic stirrer. When the chemicals were fully dissolved, the volume was made to 250 ml in a volumetric flask.

The dye solution was standardized 50 mg of ascorbic acid was dissolved in 50 ml of 0.4% oxalic acid and then 2 ml of from this solution was titrated against dye in a conical flask till the appearance of pink color. The reading of dye was noted on the burette and dye factor was calculated using the given formula:

For the calculation of ascorbic acid dilution of 10 ml of fruit juice was done in 0.4% oxalic acid prepared solution making the volume of 100 ml. Titration of 10ml from the 100ml sample solution was done against dye using liquid phenopthaline as an indicator till the persistence of light pink color. The formula used for calculation of ascorbic acid is.

Where;

F= Dye factor; T= ml of dye sol. Used from burette; D= Diluted sample (ml) taken for titration; S= fruit juice (g) taken for dilution.

Statistical analysis

The experiment was laid out in Completely Randomized Design (CRD) with two factors, repeated three times. Factor A was Aloe vera gel concentrations (0, 10, 20, 30 and 40%) and factor B was storage duration (0, 7, 14, 21, 28 and 35 days). The data was analyzed using SAS software and the treatment means were separated by LSD test as described by Steel and Torrie (1997).

 

Results and Discussion

Fruit firmness (kgcm-2)

The fruit firmness decreased significantly (P ≤ 0.05) with increasing storage duration and was retained by increasing the concentrations of Aloe vera gel. The fruit firmness of fresh (0 days of storage) fruit (3.33- 3.40 kgcm-2) decreased with incremental increase in storage duration. The lowest fruit firmness (0.56 kgcm-2) after 35 days storage at room temperature was in control fruit. The fruit firmness at 35 days storage duration was 1.3 and 1.43 kgcm-2 for fruit coated with 10 and 20% Aloe vera gel, that increased to the maximum (2.68 kgcm-2) in fruits treated with 40% gel, that was at par with fruit firmness (2.46 kgcm-2) with application of 30% gel. Furthermore, no significant changes was found in 30 and 40% gel treatment (Figure 1).

 

 

Decay incidence (%)

The interaction of storage duration and Aloe vera gel concentrations affected the fruit decay incidence significantly (P ≤ 0.05). After 7 days storage, there was no decay incidence in fruits coated with Aloe vera gel, but 12.5% decay incidence was observed in control fruits (0% gel concentration) that increased to the maximum of 100% after 35 days storage. The application of Aloe vera gel delayed the appearance of decay symptoms and decreased the decay incidence. The decay incidence was observed after 14, 21 and 28 days storage in fruits treated with 10, 20 and 30% Aloe vera gel coating. However, fruit coated 40% Aloe vera gel showed decay symptoms after 21 days storage. After 35 days storage at room temperature the maximum decay incidence (100%) was in persimmon fruit treated with 0 and 10% Aloe vera gel, that decreased to 42.25% with 30% gel application but increased to 66.66 with application of 40% Aloe vera gel (Figure 2).

 

 

Organoleptic (Taste) score

The initial organoleptic quality score of control (0 days storage) fruit (4.52) increased with increasing the storage duration to the maximum of 7.95 after 21 days storage but then decline to 7.05 and 2.55 after 28 and 35 days storage respectively. Aloe vera gel application delayed the ripening and, hence, the maximum organoleptic quality score (8.92), after 35 days storage was for fruits treated with 10% Aloe vera gel followed by fruit taste score 8.77, 7.75 and 7.33 in fruits treated with 20, 30 and 40% of Aloe gel (Figure 3).

 

 

Total soluble solids (°Brix)

The total soluble solids (TSS) of persimmon fruits was significantly (P ≤ 0.05) affected by storage intervals, concentrations of Aloe vera gel and their interaction. The interaction of the Aloe vera gel concentration and storage durations indicates that the TSS content of persimmon fruit ranged from 12.4 to 13.2% in freshly harvested fruit (day 0 of storage) that increased significantly (P ≤ 0.05) with increasing storage duration and was 37.56% after 35 days storage in control fruits (0% gel concentration). Application of Aloe vera gel retarded the increase in TSS so that it was significantly lower in gel treated fruits and was the minimum (20.2%) in fruits treated with 30% Aloe vera gel after 35 days storage (Figure 4).

Titratable acidity (%)

The storage durations, concentrations of Aloe vera gel and their interaction significantly (P ≤ 0.05) affected the titratable acidity of persimmon fruits. Titratable acidity decreased with increased in storage intervals. The titratable acidity of the fruit was 0.29-0.30% on day 0 of storage that decreased to the minimum 0.06 in control fruits after 35 days storage as compared to 0.09 and 0.11% in fruits treated with 10 and 20% Aloe vera gel respectively and stored for 35 days. The titratable acidity at the same storage duration (35 days) was the highest (0.19%) in fruit treated with 30% Aloe vera gel followed by 0.14% in fruits treated with 40% Aloe vera gel application (Figure 5).

 

 

 

Ascorbic acid (mg/100g)

The ascorbic acid content of persimmon fruits decreased with increasing storage duration and its decline was significantly (P ≤ 0.05) retarded by the application of Aloe vera gel. The ascorbic acid content of persimmon fruits was 46.33 to 46.70 mg/100g in fresh fruits (day 0 of storage) that decreased significantly (P ≤ 0.05) to 15.47 mg/100g with 35 days storage in control fruit. By contrast, the ascorbic acid content of fruit treated with 10, 20, 30 and 40% Aloe vera gel was 15.12, 17.05, 29.76 and 18.31 mg/100g accordingly (Figure 6).

 

 

The fruit firmness decreased with increasing storage duration but the decline was less than control with 30% with Aloe vera gel application (Figure 1). The loss of fruit firmness during storage of persimmon fruit is due to ripening and firmness of less than 10 N is regarded as undesirable (Salvador et al., 2004). The fruit firmness is due to cell wall strength (Vicente et al., 2007) and turgidity generated by the moisture content of the fruit (Simson and Straus, 2010). During fruit ripening, the insoluble protopectins are converted to soluble pectin and cross linked cell wall polysaccharides are degraded due to enhanced activities of polygalacturonase and pectin esterase that lead to decreased fruit firmness (Al-Juhaimi et al., 2012). Since, Aloe vera gel coating decrease the activities of polygalacturonase and pectineesterase enzymes (Vicente et al., 2007), therefore, retained the fruit firmness. The application of Aloe vera gel increases CO2 and decrease O2 inside the fruit (Salunkhe et al., 1991), which decreases the rate of respiration (Castillo et al., 2010) and ethylene production (Brishti et al., 2013) and, thus, delays ripening. Thus, the retention of fruit firmness by Aloe vera gel can also be due to modification of internal atmosphere of the fruit (Castillo et al., 2010).

The persimmon fruits are infected by variety of pathogens during storage (Khademi, 2012). Thus, the decay incidence increased with increasing storage duration due to ripening, which increases sensitivity to pathogen (Terry and Joyce, 2004). The application of Aloe vera gel delayed as well as decreased the decay incidence of persimmon fruit (Figure 2). However, fruit coating with 40% Aloe vera gel had higher decay incidence than 30% gel coating. This could be due to the complete filling up the fruit lenticel and terminating respiration by 40% of Aloe vera gel, however, 30% of Aloe vera gel only reduced the respiration rate and somehow fill up the fruit lenticel resulting in the extension of shelf life. Since, Aloe vera gel slow down the rate of ripening as well as the growth of microorganisms (Valverde et al., 2005; Mohebbi et al., 2011; Marpudi et al., 2013). Thus, Aloe vera gel application decrease disease incidence by making unfavorable environment for decay causing organism (Benitez et al., 2013).

The organoleptic quality of the fruit depends on color, taste and aroma (Aoun et al., 2013). Since the fruits continue the ripening process during storage and developed taste and color (Raza et al., 2013). The untreated persimmon (control) fruits ripened earlier during storage than the fruits treated with Aloe vera gel (Martinez-Romero et al., 2006). By contrast, the edible coating of Aloe vera gel reduces the rate of respiration and ethylene in diverse group of fruits (Chauhan et al., 2013; Guillen et al., 2013). Thus, it delayed the development of color and taste (Marpudi et al., 2013).

The total soluble solids (TSS) of persimmon fruits increased significantly with increasing storage duration but its increase was less in fruit treated with 30% Aloe vera gel (Figure 4). The total soluble solids content of the fruit contains about 75% sugars and its combinations with acidity determine the taste and flavor to the fruits. The total soluble solids content of the fruits, generally, increases during ripening and storage due to the increase in free sugars and decreased moisture content (Karki, 2005). Since, the polysaccharides contents of Aloe vera gel act as barrier against moisture loss from the fruits, thus, retard moisture loss (Misir et al., 2014) as well as delay the ripening (Ahmad et al., 2009; Brishti et al., 2013). Thus, it decreased the conversion of complex carbohydrates to free sugars (Crouch, 2003) and, thus, resulted in lower TSS in gel treated fruits.

The titratable acidity of the fruit decreased during 35 days storage but was retained at the highest value in fruit treated with 30% Aloe vera gel (Figure 5). The titratable acidity of fruit is due to different organic acid present in the fruit (Simson and Straus, 2010). Since, these organic acids are used during respiration, the titratable acidity tend to decline during storage (Jan et al., 2015). The application of Aloe vera gel decreases the rate of respiration, thus, decrease the loss of organic acids and retain higher titratable acidity (Ahmad et al., 2009). Aloe vera gel acts as a barrier for the exchange of gases and increases the internal CO2 concentration and modify the internal atmospheric of the fruit that retard the ripening and decreases the decline of titratable acidity (Ergun and Satici, 2012).

The ascorbic acid content of persimmon fruits decreased significantly during storage in control fruit but was higher than control in fruits treated with Aloe vera gel (Figure 6). The ascorbic acid contents of the fruit decease with increasing storage duration (Jan et al., 2015) due to enhanced activity of ascorbic acid oxidase enzyme (Salunkhe et al., 1991). Aloe vera gel application on pineapple and oranges delayed the loss of ascorbic acid by decreasing the permeability of oxygen into the fruits (Misir et al., 2014) and so preventing the oxidation of ascorbic acid (Adetunji et al., 2012). It is however, interesting to observe that high gel concentration (40%) caused significant reduction in ascorbic acid, probably by increasing internal CO2 levels that trigger ascorbic acid degradation (Lee and Kader, 2000).

 

Conclusions and Recommendations

It was concluded that persimmon fruit treated with 30% Aloe vera gel, as edible coating, ripened slowly during storage. Aloe vera gel application (30%) retarded the increase in TSS and the loss of acidity and ascorbic acid as well as retained firmness. It also decreased the decay incidence and retained high organoleptic quality. However, lower (10 and 20%) and high gel concentrations (40%) were less effective in increasing the storage life and retaining quality of persimmon fruit.

 

Acknowledgments

This work is supported by Professor Dr. Abdur Rab, Chairman of the Department of Horticulture, Faculty of Crop Production Sciences, University of Agriculture, Peshawar 25130, Khyber Pakhtunkhwa, Pakistan during 2013-15.

 

Novelty Statement

The study is novel for fruit growers and preservwers as persimmon fruits treated with 30% Aloe vera gel retains post-harvest quality of fruits. It also preserve it from deterioration for up to ≥ 35 days of storage at room temperature.

 

Author’s Contribution

The manuscript is based on the M.Sc (Hons) degree research work of Abdul Jalal under the supervision of Professor Dr. Abdur Rab, Abdul Jalal and Naveed Ahmad designed and performed the experiment. Abdur Rab analysed the results and edit the original draft of the manuscript.

Conflicts of interest

The authors declare no conflicts of interest.

 

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