Globally, an increased use of pesticides has resulted in the occurrence of drug resistant microbes. This has urged the investigators and researchers to use and develop the antimicrobial agents derived from some natural resources. Among various naturally occurring medicinal plants, Cinnamaldehyde, owing to its acid generation, acid tolerance and virulence gene expression has gained a considerable attention. Keeping in view its beneficial aspects, the present study has been conducted to investigate the contents and compare the nutritional and antimicrobial activities of Cinnamon collected from various regions of Asia. In this study, antimicrobial activity of cinnamon was estimated using well diffusion method against various bacterial and fungal strains. As shown by the findings of the present study, Cinnamon oil is found a potent antibacterial agent against all microbes used. Additionally, it was found that cinnamon samples collected from Sri Lanka has the highest oil content (3.1%), followed by China (2.8 %) and India (1.9 %), where all tests were standardised using fixed oil levels. In terms of antimicrobial properties, obtained results demonstrate that Indian cassia (Cinnamomum tamala) exhibited the highest antimicrobial activity as compared to the samples collected from other regions. The findings can be beneficial in establishing a synergistic relation to the extent of oil content and its combating properties to the microorganisms in various species of cinnamon.