Nuclear and Cytoplasmic Maturation of In Vitro Matured Denuded Oocytes in the Presence of Gonadotrophins, Oestradiol and Insulin in Nili Ravi Buffalo
Nuclear and Cytoplasmic Maturation of In Vitro Matured Denuded Oocytes in the Presence of Gonadotrophins, Oestradiol and Insulin in Nili Ravi Buffalo
Beenish Shahid1* and Muhammad Ijaz Khan2
Fig. 1.
Photomicrographs of fully grown Nili Ravi buffalo oocytes during in vitro culture: (a) An intact germinal vesicle (indicated by arrow) with a single prominent nucleus arrested in dictyate stage of the first meiotic prophase. (b) Oocyte undergoing GVBD showing complete dissolution of the GV with only a small remnant of the nucleolus. (c) MII oocyte arrested at metaphase II stage and omitted a polar body (indicated by arrow). (d) Degenerated oocyte showing shrinkage of ooplasm. Photographs were taken from an inverted phase contrast (Nikon) microscope (objectives: a-d, X20).
August 2023
Vol. 55, Iss. 4, Pages 1501-2000
