Molecular Detection of β-lactamase Genes and Antibiotics Resistance for Bacterial Isolates From Pigeon’s Eyes Infections
Molecular Detection of β-lactamase Genes and Antibiotics Resistance for Bacterial Isolates From Pigeon’s Eyes Infections
Ali Abd Kadhum
ABSTRACT
The inflammation of the inner surface of the eyelid and the outermost layer of the white portion of the eye is known as conjunctivitis, or pink eye. In one or both eyes, conjunctivitis can occur. A total of 89 pigeons include In this study, eye swabs were taken from pigeons suffering from eye inflammation, and then the swabs were cultured on MacConkey agar and Blood agar. The pigeons’ eyes swabs taken from were cultured by a streak method and incubator with a 37C. For 24 hours. 10 samples with no growth appear, and 79 isolates diagnosed the bacterium depending on the appearance qualities of the developing colonies, pigmentation In Gram stain, as well as biochemical examinations. Different bacterial species was confirmed using the Enterosystem 18 R system and using a device. Vitek-2 compact system. Results of an antibiotic resistance test showed that the isolates showed a “significant” change in resistance to antibiotics, as the isolates showed the bacterial species Staphulococcu aureus, Pseudomonas aeruginosa, Streptococcus pneumonia, Moraxilla catarrhalis, and Klebsiella pneumonia resistance (100%) to the antibiotic Amoxycillin. While isolates of the bacterial species Staphulococcu aureu, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, and Proteus vulgaris showed resistance (100%) to the antibiotic azithromycin. Klebsiella pneumonia and E.coli isolates showed resistance to the antibiotic Moxifloxacin (90%), while Staphylococcus aureus isolates showed resistance to the antibiotic ciprofloxacin (90%). The BlaTEM gene for seven isolates E.coli causing conjunctivitis infection were successfully amplified. While BlaCTX-M gene for all proteus vulgaris isolates. As well as BlaSIM gene for isolates P. aeroginosa and found one isolate only carry BlaSIM gene. showed five isolates staph. aureus encode PVL gene
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