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Incidence of blaIMP and blaVIM Genes among Carbapenemase Producing Escherichia coli in Lahore, Pakistan

Incidence of blaIMP and blaVIM Genes among Carbapenemase Producing Escherichia coli in Lahore, Pakistan

 Shafqat Husnain Khan1, Shah Jahan2, Irshad Ahmad3,4, Sadeeq ur Rahman5,* and Tayyab ur Rehman4,*

1Department of Pathology, Microbiology Section, Continental Medical College, Lahore, Pakistan
2Department of Immunology, University of Health Sciences, Lahore, Pakistan
3School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
4Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan
5College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Pakistan

*     Corresponding author: sadeeq@awkum.edu.pk;

tayyab.ibms@kmu.edu.pk

 

ABSTRACT
Antibiotic drug resistance in E. coli is a worldwide health problem. Particularly, increasingly reported carbapenem resistance due to carbapenemases is highly worrisome. Literature regarding incidence of class B metallo-β-lactamases (MBLs) - producing E. coli has not yet judiciously been addressed. In this study, we investigated the occurrence of carbapenemase- and MBLs-producers among a collection of 100 carbapenem resistant E. coli obtained from a tertiary hospital at Lahore Pakistan. All carbapenemase producers were further investigated to identify frequency of blaIMP and blaVIM encoding genes. Results of modified hodge test identified 81% carbapenemase producers, while combined disc diffusion test identified 28 isolates as MBL producers among them. Of all carbapenemase producers, 25 isolates (30.8%) of carbapenemase-producing E. coli were found to harbor bla-VIM gene, while 13 isolates (16.04%) were found to carry blaIMP gene. We report on high incidence rate of carbapenemase producers among carbapenem resistant E. coli isolates.

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Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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