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Bacillus subtilis BML5 Isolated from Soil Contaminated with Poultry Waste has Keratinolytic Activity

Bacillus subtilis BML5 Isolated from Soil Contaminated with Poultry Waste has Keratinolytic Activity

Aqsa Imtiaz and Abdul Rehman*

Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan

*      Corresponding author: rehman_mmg@yahoo.com

 

Fig. 1.

Growth curves of B. subtilis BML5 in medium containing glucose (control) and in mineral salt medium supplemented with 1% feathers as substrate (treated) incubated at 37°C.

Fig. 2.

Effect of temperature (A), pH (B) and metal ions (C) on keratinase activity isolated from B. subtilis BML5.

Fig. 3.

Coomassie Brilliant blue-stained SDS-PAGE of partially purified keratinase from B. subtilis BML5. Lane C, bacterium growing without feather; Lane T, bacterium growing with feather; Lane M, molecular mass protein maker.

Fig. 4.

Degradation of feathers by B. subtilis BML5 culture in minimal salt medium after 6 days of incubation at its optimum temperature.

Pakistan Journal of Zoology

October

Vol. 50, Iss. 5, Pages 1601-1998

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