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Profiling of Apoptosis-Related Genes in Erythrocytes of Chickens Infected with Avian Influenza Virus (H9N2 Subtype)

Profiling of Apoptosis-Related Genes in Erythrocytes of Chickens Infected with Avian Influenza Virus (H9N2 Subtype)

Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Qian-qian Cheng1
Raza Ali Mangi1, Ali Raza Jahejo1, Afrasyab Khan1, Yu-hai Bi1,2 and Wen-xia Tian1*

1College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China
2CAS Key Laboratory of Pathogenic Microbiology and Immunology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China

*      Corresponding author: wenxiatian@126.com

Fig. 1.

Total volume used in control and experimental groups and their respective repeat groups at 0, 2, 6 and 10 h of H9N2 infection.

Fig. 2.

mRNA expression of apoptosis-related genes in chicken’s erythrocytes at different time intervals (0, 2, 6 and 10 h). Asterisks (*) indicating significant differences among experimental and control groups (*p < 0.05; **p < 0.01; ***p < 0.0004; ****p < 0.0001; ns, not significant) C, control; EXP, experimental.

Fig. 3.

Protein expression of Bax in chicken’s erythrocytes at different time intervals of 0, 2, 6 and 10 h (Protein bands derived from Western blot). Data represent mean±SEM. Asterisks (*) indicating significant differences compared to control groups (*p < 0.05; **p < 0.01; ***p < 0.0004; ****p < 0.0001; ns, not significant) C, control; EXP, experimental.

Pakistan Journal of Zoology

December

Vol. 53, Iss. 6, Pages 2001-2521

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