Morphological and Molecular Characterization of Rumen Fluke Species from Sheep in Southeastern Pakistan
Mahvish Rajput1, Abdullah G. Arijo1, Muhammad Bachal Bhutto1, Rehana Shahnawaz Buriro2, Javaid Ali Gadahi1, Muhammad Naeem3 and Zubair Ahmed Laghari1*
Sequence alignments of ITS2 sequences of isolate of present study. Base homologies are indicated with dots (.), gaps are indicated with hyphens (-), and base changes are indicated with the substituted base.
The phylogenetic tree analysis of ITS2 region of rDNA sequences of different trematodes species. The rumen fluke isolates collected from native sheep of Hyderabad, Sindh province, Pakistan shows the isolates were closely related to Paramphistumum epiclitum and evolutionary conservation between different trematode species.
The multiple sequence alignment of rumen fluke isolates identified in the current study (AMPHI-HYD) and other related species. (Calicophoron shillongensis; Paramphistomum cervi; Paramphistomum leydeni; Gastrothylax crumenifer; Paramphistomum epiclitum; Fischoederius elongatus).