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Molecular Phylogeny and Diversity Analysis of Hog Deer (Axis porcinus) in Pakistan

Molecular Phylogeny and Diversity Analysis of Hog Deer (Axis porcinus) in Pakistan

Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1

1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
2Department Molecular Biology, Virtual University of Pakistan, Lahore, Pakistan
3Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand, Pakistan

*      Corresponding author: asifnadeem@uvas.edu.pk

ABSTRACT

Many species of mammals have declined within the past two centuries due to human caused disturbances and the unsustainable use of natural resources. Molecular methods have an important role in phylogeny and diversity analysis. In Pakistan hog deer (Axis porcinus) is an important wild species belongs to the family Cervidae. The hog deer is one of the least studied species at molecular level in Pakistan. The present study was planned to investigate its molecular phylogeny and diversity analysisusing mitochondrial cytochrome b, cytochrome c and d-loop region. Samples werecollected from different localities. After DNA extraction and quantification, amplification of gene was done by polymerase chain reaction. PCR products were sequenced bi-directionally, aligned and single nucleotide polymorphisms were identified. Bioinformatics tools were applied for construction of phylogenetic tree and genetic diversity analysis. Lower genetic diversity was observed. The finding of this research is prerequisite for future research.
 

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Pakistan Journal of Zoology (Associated Journals)

August

Vol. 49, Iss. 4, Pages 1151-1546

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