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Molecular Characterization of Laboratory Reared Glossina palpalis palpalis and Glossina morsitans submorsitans Populations in Nigeria

Molecular Characterization of Laboratory Reared Glossina palpalis palpalis and Glossina morsitans submorsitans Populations in Nigeria

Christopher U Orji1*, Ignatius O Onyeocha2, Steven S Shaida3, Peter M Dede4, Bitrus Yakubu5, Elijah E Ella6 and Pam D Luka5

1Department of Agricultural Biotechnology, National Biotechnology Development Agency, Abuja, Nigeria; 2Department of Biotechnology, Federal University of Technology, Owerri, Nigeria; 3Vector and Parasitology Studies Department, Nigerian Institute for Trypanosomiasis (and Onchocerciasis) Research (NITR), Kaduna, Nigeria; 4Pan African Tsetse and Trypanosomiasis Eradication Campaign (PATTEC), Nigerian Institute for Trypanosomiasis (and Onchocerciasis) Research (NITR), Kaduna, Nigeria; 5Applied Molecular Biology Division, Nigerian Veterinary Research Institute (NVRI), Vom, Jos, Nigeria; 6Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria.

Email: orjiuchristopher@yahoo.com

ABSTRACT

Tsetse fly (Diptera: Glossinidae) vector control measures can rely upon knowledge of genetic diversity between species’ population. Two species were studied: Glossina palpalis palpalis and Glossina morsitans submorsitans using two mitochondrial DNA fragment primers- Cytochrome oxidase subunit II (COII) and Cytochrome b (CytB). Twelve samples of laboratory-reared population of each species were used for the study. Sequencing data were used to calculate haplotype, haplotype diversity and nucleotide diversity for the two species. Based on combined loci of cytochrome oxidase II (COII) and cytochrome b (CytB), twelve haplotypes were generated for G. p. palpalis and five for G. m. submorsitans. Similarly, for the combined loci, haplotype diversity (Hd) was higher for G. p. palpalis (0.90) than G. m. submorsitans (0.68). Nucleotide diversity was homogenous for G. m. submorsitans (0.24859) than G. p. palpalis (0.24093). A higher genetic diversity suggests a higher evolutionary rate. Haplotype diversity and Nucleotide diversity (genetic diversity) of a species population is often related to the ecological systems, evolutionary rate, adaptation of the species, gene flow, epidemiology and control strategies. Our study observed that the two laboratory grown species of tsetse flies may be genetically related.

 

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Veterinary Sciences: Research and Reviews

June

Vol. 5, Iss. 1, Pages 1-32

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