Submit or Track your Manuscript LOG-IN

LncRNA BMF-AS1 Exerts Anti-Apoptosis Function in COPD by Regulating BMF Expression

LncRNA BMF-AS1 Exerts Anti-Apoptosis Function in COPD by Regulating BMF Expression

Chuntao Li1, Hua Liu2, Jianqing Zhang1*, Jiaqiang Zhang1, Luming Dai1, Zhihuan Zhao1,Lizhou Fang1, Ling Liu1, Jingkui Shu1 and Jiagang Feng1

1Department of Respiratory Critical Care Medicine, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
2Department of Clinical Laboratory, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, China

*      Corresponding author: ydyyzjq@163.com

 

ABSTRACT

Previous studiesindicate that long non-coding RNAs (lncRNAs) have crucial effect in COPD, but the exact role of most lncRNAs in COPD remains unkown. We detected differential expression of mRNAs and lncRNAs in blood samples from COPD patients (n=3) and healthy controls (n=3) with smoking history by human microarray analysis. Then, we selected lncRNAs and their associated mRNAs which were both differential expression in COPD vs healthy smokers, for quantitative reverse transcription PCR (qRT-PCR). A total of 3,359 lncRNAs revealed by microarray analysishave been differentially expressed between the two groups, 1,995 lncRNAs identified to be up-regulated in COPD, whereas 1,364 lncRNAs have been down-regulated. Meanwhile,a total of 3,283 mRNAs have been differentially expressed, 2,589 mRNAs identified to be up-regulated in COPD, whereas 694 mRNAs were down-regulated. qRT-PCR showed there was a statistically significant difference between COPD and control group for BMF (p=0.0121), lncRNA RP11-521C20.3 (p<001), CYLD (p=0.003) and lncRNA RP1-85F18.5 (p=0.005). Both the results of microarray analysis and qRT-PCR validation test has showed that lncRNA RP11-521C20.3 (BMF antisense RNA 1, BMF-AS1) significantly decreased, while it’s associated BMF mRNA increased in COPD. This study indicates that the expression of genome-wide lncRNA in COPD blood samplesis different in comparison with healthy smokers by microarray analysis. LncRNA BMF-AS1 expression might potentially predict the apoptosis of COPD by regulating of BMF mRNA expression.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

June

Vol. 52, Iss. 3, Pages 825-1224

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe