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Sequence Amplicon Analysis of 18S rDNA Variable Regions (V3, V4 and V9) in Natural Strains of Sordaria fimicola and Response to Different Carbohydrates

Sequence Amplicon Analysis of 18S rDNA Variable Regions (V3, V4 and V9) in Natural Strains of Sordaria fimicola and Response to Different Carbohydrates

Rabia Arif*, Shahar Bano, Muhammad Ishfaq and Muhammad Saleem

Molecular Genetics Research Laboratory, Botany Department, University of the Punjab, Lahore 54590, Pakistan

*      Corresponding author: phdgenetics@gmail.com

Fig 1

V3 and V4 region analysis of 18S rRNA genes in natural strains of S. fimicola and their F1 progeny. M, DNA marker; S3, parental strain; 1, 2 and 3, F1 progeny of S3; N5, parental strain; 4, 5, 6 and 7, F1 progeny of N5; Neg., negative control without genomic DNA

Fig 2

CLUSTAL O (1.2.3) multiple sequence alignment of different strains of Sordaria fimicola with reference sequence of S. fimicola.

Fig 3

Circular Phylogram to find phylogenetic relationship among ascomycetes and experimental strains reconstructed from the conserved region spanning V3 and V4 rDNA genes (A) and V9 rDNA genes (B) yellow highlighted colour indicating strain under study.

Pakistan Journal of Zoology (Associated Journals)

October

Vol. 49, Iss. 5, Pages 1547-1936

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