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Ganoderma lucidum as a Biocontrol agent for Management of Alternaria solani, A Pathogen of Early Blight of Tomato

Ganoderma lucidum as a Biocontrol agent for Management of Alternaria solani, A Pathogen of Early Blight of Tomato

Muhammad Asif1*, Ahmad Ali Shahid1,2 and Nasir Ahmad3

1Insititute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan; 2Center of Excellence and Molecular Biology, University of the Punjab, Lahore, Pakistan; 3School of Biological Sciences, University of the Punjab Lahore, Pakistan.

 
*Correspondence | Muhammad Asif, Insititute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan; Email: ali_asif514@yahoo.com

ABSTRACT

Tomato is a beneficial, economically important and highly consumable crop. Several fungal diseases may affect its yield. Early blight is one of its most destructive diseases. Biocontrol agents are the best-known source to minimize the attack of pathogen. Tomato leaves infected with early blight symptoms were collected from Lahore. Pathogen was isolated on PDA and its morphological characters were studied. All the characters used for identification of pathogen confirmed the culture as Alterneria solani. Ganoderma lucidum was collected from University of the Punjab, Lahore associated with dead oaks of Dalbergia sisso. Macro and microscopic identification was performed to confirm the characteristics of G. lucidum. Interaction of G. lucidum with A. solani was also observed to measure the disease inhibiting potential. Crude protein extract was also prepared to observe the inhibitory effect of G. lucidum against A. solani. The results for interaction of mycelia of G. lucidum with pathogen A. solani reveal that G. lucidum is an efficient biocontrol agent to reduce the disease incidence. Crude protein extract was also found beneficial to control the pathogen. The results for minimum inhibitory concentration (MIC) of crude protein extract reveal that 5mg/ml is the concentration, which inhibited 100% growth of A. solani. It was concluded that either mycelia or crude protein extract of G. lucidum could be used for pathogen inhibition.
 

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Sarhad Journal of Agriculture

June

Vol. 38, Iss. 2, Pages 388-758

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